藏红花侧芽中L-瓜氨酸的提取与测定
Extraction and determination of L-Citrulline in the lateral bud of saffron
作者：李数数(四川大学生命科学学院 生物资源与生态环境教育部重点实验室)；王静(四川大学生命科学学院 生物资源与生态环境教育部重点实验室)；侯静(四川大学生命科学学院 生物资源与生态环境教育部重点实验室)；彭彤(四川大学生命科学学院 生物资源与生态环境教育部重点实验室)；何韵云(四川大学生命科学学院 生物资源与生态环境教育部重点实验室)；魏琴(宜宾学院 香料植物资源开发与利用四川省高校重点实验室)；白洁(四川大学生命科学学院 生物资源与生态环境教育部重点实验室)
Author：LI Shu-Shu(Key Laboratory Bio-resources and Eco-environment of Ministry Education,College of Life Sciences,Sichuan University)；WANG Jing(Key Laboratory Bio-resources and Eco-environment of Ministry Education,College of Life Sciences,Sichuan University)；HOU Jing(Key Laboratory Bio-resources and Eco-environment of Ministry Education,College of Life Sciences,Sichuan University)；PENG Tong(Key Laboratory Bio-resources and Eco-environment of Ministry Education,College of Life Sciences,Sichuan University)；HEYun-Yun(Key Laboratory Bio-resources and Eco-environment of Ministry Education,College of Life Sciences,Sichuan University)；WEI Qin(Key Lab of Aromatic Plant Resources Exploitation and Utilization in Sichuan Higher Education, Yibin University,)；BAI Jie(Key Laboratory Bio-resources and Eco-environment of Ministry Education,College of Life Sciences,Sichuan University)
收稿日期：2017-12-05 年卷（期）页码：2018,55(6):1297-1300
期刊名称：四川大学学报: 自然科学版
Journal Name：Journal of Sichuan University (Natural Science Edition)
关键字：藏红花侧芽；L-瓜氨酸；超高效液相色谱法；提取；含量测定
Key words：Lateral bud; L-citrulline; Ultra-high performance liquid chromatography; Extraction; Content determination
基金项目：四川省高校重点实验室开放基金（2016XLZ002）；西红花规模化生产技术服务（HS20100601106684）
中文摘要
对藏红花（Crocus sativus L.）侧芽中的L 瓜氨酸进行定性定量分析，并对提取方法进行优化.样品采用超声波法提取，2,4-二硝基氟苯（FDNB）柱前衍生，通过超高效液相色谱进行分析.实验结果表明L-瓜氨酸在0.98~62.50μg/mL（R2= 0.9998）范围内线性关系良好，检测限（S/N=3）0.2440ng/mL，样品平均回收率为101.15% (n=6,RSD=1.00% ).藏红花侧芽中含有丰富的L 瓜氨酸，提取条件经优化后，测得藏红花侧芽中L 瓜氨酸的含量约为26.69 mg/g.
英文摘要
L-citrulline in the lateral bud of saffron was quantitatively analyzed for the first time and the extraction method was optimized. After ultrasonic extraction, the samples were derived with 2,4-dinitrofluorobenzene (FDNB) and analyzed by Ultra High Performance Liquid Chromatography. The results showed that a good linear relationship was obtained for L-citrulline in the concentration range of 0.98~62.50 μg/mL with R2 = 0.9998,the detection limit of L-citrulline was 0.2440ng/mL（S/N=3）, and the average recovery was 101.15% ( n=6, RSD=1.00% ). After the extraction method was optimized,the content of L-citrulline in the lateral bud of saffron was determined to be about 26.69 mg/g.
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