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论文摘要

唐古特大黄内生放线菌分离鉴定及生防作用研究

Isolation of Endophytic Actinomycetes from Rheum tanguticum and Its Application in Biogical Control of Magnaporthe grisea

作者:廖敏(四川农业大学资源学院; 绵阳师范学院生命科学与技术学院);张波(四川农业大学资源学院);范中菡(四川农业大学资源学院);陈熊春蕊(四川农业大学资源学院);张小平(四川农业大学资源学院)

Author:LIAO Min(College of Resources, Sichuan Agricultural University; College of Life Science&Biotechnology, Mianyang Normal University);ZHANG Bo(College of Resources, Sichuan Agricultural University);FAN Zhong-Han(College of Resources, Sichuan Agricultural University);CHEN Xiong-Chun-Rui(College of Resources, Sichuan Agricultural University);ZHANG Xiao-Ping(College of Resources, Sichuan Agricultural University)

收稿日期:2016-03-04          年卷(期)页码:2017,54(2):381-386

期刊名称:四川大学学报: 自然科学版

Journal Name:Journal of Sichuan University (Natural Science Edition)

关键字:药用植物;内生放线菌;稻瘟病菌;生物防治

Key words:Medicinal plant;Endophytic actinomycetes;Magnaporthe grisea;Biological control

基金项目:国家高技术研究发展计划

中文摘要

以唐古特大黄为材料,采用匀浆涂布法进行内生放线菌的分离,并运用平板对峙法和生长速率法筛选内生放线菌的抑菌活性,最终获得一株具有抑制稻瘟病菌活性的内生放线菌4-21。通过形态学观察、生理生化特性检测和16S rDNA序列分析鉴定菌株4-21为Streptomyces albidoflavus 微白黄链霉菌;通过盆栽试验验证菌株4-21对稻瘟病菌的防治效果。结果表明,内生放线菌4-21在水稻稻瘟病的生物防治中具有潜在的应用价值。

英文摘要

Endophytic actinomycetes were isolated from Rheum tanguticum by spread plate method. Inhibitory activities of endophytic actinomycetes were determined by dual-culture inhibition on agar plate and growth rate method. As a result, Strain 4-21with high inhibitory effect on Magnaporthe grisea was found. The strain 4-21 was indentified as Streptomyces albidoflavus according to the morphology biochemical characterization and 16S rDNA gene sequence analysis. And its potted control effect was also investigated. Strain 4-21 deserved to be developed a kind of biocontrol agent.

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