瑞香狼毒对东亚飞蝗作用机理的初步研究
Studies on the mechanism of Stellera chamaejasme L. to Locusta migratoria manilensis (Meyen)
作者：陈琼(四川大学生命科学学院生物资源与生态环境教育部重点实验室)；高甜甜(四川大学生命科学学院生物资源与生态环境教育部重点实验室)；李波(四川大学生命科学学院生物资源与生态环境教育部重点实验室)；查艳梅(四川大学生命科学学院生物资源与生态环境教育部重点实验室)；陶科(四川大学生命科学学院生物资源与生态环境教育部重点实验室)；金洪(四川大学生命科学学院生物资源与生态环境教育部重点实验室)；侯太平(四川大学生命科学学院生物资源与生态环境教育部重点实验室)
Author：CHEN Qiong(Key Laboratory of Bio-resources and Eco-environment of Ministry of Education, College of Life Sciences, Sichuan University)；GIAO Tian-Tian(Key Laboratory of Bio-resources and Eco-environment of Ministry of Education, College of Life Sciences, Sichuan University)；LI Bo(Key Laboratory of Bio-resources and Eco-environment of Ministry of Education, College of Life Sciences, Sichuan University)；ZHA Yan-Mei(Key Laboratory of Bio-resources and Eco-environment of Ministry of Education, College of Life Sciences, Sichuan University)；TAO Ke(Key Laboratory of Bio-resources and Eco-environment of Ministry of Education, College of Life Sciences, Sichuan University)；JIN Hong(Key Laboratory of Bio-resources and Eco-environment of Ministry of Education, College of Life Sciences, Sichuan University)；HOU Tai-Ping(Key Laboratory of Bio-resources and Eco-environment of Ministry of Education, College of Life Sciences, Sichuan University)
收稿日期：2016-03-16 年卷（期）页码：2017,54(3):665-669
期刊名称：四川大学学报: 自然科学版
Journal Name：Journal of Sichuan University (Natural Science Edition)
关键字：瑞香狼毒；东亚飞蝗；Na+-K+-ATPase；Ca2+/Mg2+-ATPase；乙酰胆碱酯酶
Key words：Stellera chamaejasme L.; Locusta migratoria manilensis (Meyen); Na+-K+-ATPase; Ca2+/Mg2+-ATPase; acetylcholinesterase
基金项目：国家自然科学基金(31272068) ；四川省应用基础研究项目（2014JY0063）
中文摘要
利用硅胶柱分离瑞香狼毒乙酸乙酯萃取物得到I、II、III段粗分物，并测定对东亚飞蝗毒杀活性最高的I段粗分物对东亚飞蝗的致死性及对其体内Na+-K+-ATPase、Ca2+/Mg2+-ATPase、乙酰胆碱酯酶的抑制活性. 研究表明：瑞香狼毒I段粗分物对东亚飞蝗具有较强的毒杀作用，在药物浓度为10mg/mL、72h对东亚飞蝗的校正死亡率为92.5%；同时，毒杀作用具有浓度和时间依赖性；而且瑞香狼毒乙酸乙酯萃取物I段粗分物对上述3种酶均有一定的抑制作用，且对Na+-K+-ATPase的抑制作用最强，在药物浓度为5mg/mL、96h对Na+-K+-ATPase的抑制率为40.35%；分析发现瑞香狼毒活性成分对东亚飞蝗的毒杀作用与对Na+-K+-ATPase的抑制作用之间具有显著相关性，据此推测，Na+-K+-ATPase可能是瑞香狼毒活性成分的主要作用靶标.
英文摘要
Using silica gel column to separate the ethyl acetate extract of Stellera chamaejasme L. we can get I, II, III sections. The activity of Na+-K+-ATPase、Ca2+/Mg2+-ATPase、acetylcholinesterase in insects were determined in vivo. The results showed that I section from Stellera chamaejasme L. had the strongest poison effect on Locusta migratoria manilensis (Meyen). Under the concentration of 10 mg/mL, the corrected mortality was 92.5% in 72 hour. And the poison effect there was persistence. The I section from Stellera chamaejasme L. also had certain inhibition on activity of Na+-K+-ATPase、Ca2+/Mg2+-ATPase、acetylcholinesterase. The inhibiton rate of Na+-K+-ATPase was the highest among the three kinds of enzymes. There was a well correlation between the toxic action of the active ingredients from Stellera chamaejasme L. to Locusta migratoria manilensis (Meyen) and the inhibition on activity of Na+-K+-ATPase in Locusta migratoria manilensis (Meyen). Na+-K+-ATPase is likely the target of the active ingredients from Stellera chamaejasme L. against Locusta migratoria manilensis (Meyen).
【关闭】
