拟南芥蛋白质激酶CARK7与ABA受体RCAR12相互作用的研究
The research on interaction between the Protein kinase CARK7 and the ABA receptor RCAR12 in Arabidopsis
作者：李晶祥(四川大学生命科学学院生物资源与生态环境教育部重点实验室)；李小意(四川大学生命科学学院生物资源与生态环境教育部重点实验室)；黄琪(四川大学生命科学学院生物资源与生态环境教育部重点实验室)；陈静波(四川大学生命科学学院生物资源与生态环境教育部重点实验室)；李旭锋(四川大学生命科学学院生物资源与生态环境教育部重点实验室)；刘志斌(四川大学生命科学学院生物资源与生态环境教育部重点实验室)；杨毅(四川大学生命科学学院生物资源与生态环境教育部重点实验室)
Author：LI Jing-Xiang(Key Laboratory of Bio-resources and Eco-environment of Ministry of Education, College of Life Sciences, Sichuan University)；LI Xiao-Yi(Key Laboratory of Bio-resources and Eco-environment of Ministry of Education, College of Life Sciences, Sichuan University)；HUANG Qi(Key Laboratory of Bio-resources and Eco-environment of Ministry of Education, College of Life Sciences, Sichuan University)；CHEN Jing-Bo(Key Laboratory of Bio-resources and Eco-environment of Ministry of Education, College of Life Sciences, Sichuan University)；LI Xu-Feng(Key Laboratory of Bio-resources and Eco-environment of Ministry of Education, College of Life Sciences, Sichuan University)；LIU Zhi-Bin(Key Laboratory of Bio-resources and Eco-environment of Ministry of Education, College of Life Sciences, Sichuan University)；YANG Yi(Key Laboratory of Bio-resources and Eco-environment of Ministry of Education, College of Life Sciences, Sichuan University)
收稿日期：2017-01-18 年卷（期）页码：2017,54(5):1077-1082
期刊名称：四川大学学报: 自然科学版
Journal Name：Journal of Sichuan University (Natural Science Edition)
关键字：蛋白质激酶；相互作用；CARK7；RCAR12
Key words：Protein kinase; Interaction; CARK7; RCAR12
基金项目：国家自然科学基金；973计划
中文摘要
脱落酸（ABA）受体RCARs在ABA信号传导过程中起关键作用，研究ABA受体的相互作用蛋白质对进一步了解植物抗逆的分子机制有重要的意义。通过酵母双杂交和GST-pull down的方法在体外研究蛋白质激酶CARK7与ABA受体RCAR12之间的相互作用，结果表明CARK7与RCAR12在体外具有明显的相互作用。进一步通过双分子荧光互补实验，证明CARK7与RCAR12在烟草叶片表皮细胞中同样具有明显的相互作用。因此，本研究通过体外和体内两个方面证明了CARK7与RCAR12之间的相互作用，为进一步研究ABA信号转导的调控奠定了基础。
英文摘要
The ABA receptor RCARs play a key role in ABA Signal transduction process, and research on interaction proteins of RCARs has great significance in further understanding the molecular mechanism of plants in adverse environment. The yeast two-hybrid assay and GST-pull down were used to study the interaction between CARK7 and RCAR12. The results showed that there is a significant interaction between CARK7 and RCAR12 in vitro. Then, the bimolecular fluorescence complementation (BiFC) was used to confirm the interaction between CARK7 and RCAR12 in vivo. The BiFC assay indicated that CARK7 was associated with RCAR12 in tobacco epidermal cells. Taken together, these data suggested that CARK7 and RCAR12 closely interact with each other, and illuminating the regulation of the ABA signaling transduction.
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