猪Smad7和Smad9基因克隆、序列分析与组织表达图谱探究
Molecular cloning, sequence analysis and tissue distribution of pig Smad7 and Smad9 genes
作者：陈巧容(四川大学生命科学学院生物资源与生态环境教育部重点实验室)；李娟(四川大学生命科学学院生物资源与生态环境教育部重点实验室)；王亚军(四川大学生命科学学院生物资源与生态环境教育部重点实验室)
Author：CHEN Qiao-Rong(Key laboratory of Bio resources and Eco environment of Ministry of Education,College of Life Sciences, Sichuan University)；LI Juan(Key laboratory of Bio resources and Eco environment of Ministry of Education,College of Life Sciences, Sichuan University)；WANG Ya-Jun(Key laboratory of Bio resources and Eco environment of Ministry of Education,College of Life Sciences, Sichuan University)
收稿日期：2013-04-19 年卷（期）页码：2015,52(1):157-162
期刊名称：四川大学学报: 自然科学版
Journal Name：Journal of Sichuan University (Natural Science Edition)
关键字：猪; Smad7; Smad9; 分子克隆; 组织表达
Key words：Pig; Smad7; Smad9; Cloning; Tissue expression
基金项目：国家重大专项转基因生物新品种培育(2009zx08009145B)
中文摘要
本研究采用RT-PCR方法, 从长白猪脑中克隆了Smad7和Smad9的基因cDNA全长. 序列比对发现Smad7和Smad9在不同物种中保守性很高. 同时, RT-PCR检测发现: Smad7和Smad9在家猪各组织中广泛表达. 这些结果提示: Smad7和Smad9可参与家猪众多生理过程的调节
英文摘要
In this study, Smad7 and Smad9 genes were cloned from brain tissues of Landrace pigs. Amino acid sequence comparison shows that they all exhibit remarkable degree of amino acid sequence identity (>97%) with those of other vertebrates. RT-PCR assay showed that the mRNA of Smad7 and Smad9 are widely distributed in various tissues of pig, suggesting that these conserved proteins may play critical roles in cellular signaling and participate in a number of physiological processes of pigs.
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