CdTe量子点对人乳腺癌细胞MCF 7/ADM的化学增敏作用及其机制研究
Mechanisticanalysis of CdTe quantum dots enhanced chemosensitization of MCF 7/ADM cells
作者：钟文英(四川大学生命科学学院)；李晓明(中国科学院上海应用物理研究所)；韩玉萍(四川大学生命科学学院)；陈楠(中国科学院上海应用物理研究所)；黄庆(中国科学院上海应用物理研究所)；赵云(四川大学生命科学学院)
Author：ZHONG Wen-Ying(College of Life Sciences, Sichuan University)；LI Xiao-Ming(Laboratory of Physical Biology, Shanghai Institute of Applied Physics, Chinese Academy of Sciences)；HAN Yu-Ping(College of Life Sciences, Sichuan University)；CHEN Nan(Laboratory of Physical Biology, Shanghai Institute of Applied Physics, Chinese Academy of Sciences)；HUANG Qing(Laboratory of Physical Biology, Shanghai Institute of Applied Physics, Chinese Academy of Sciences)；ZHAO Yun(College of Life Sciences, Sichuan University)
收稿日期：2013-11-21 年卷（期）页码：2015,52(2):397-402
期刊名称：四川大学学报: 自然科学版
Journal Name：Journal of Sichuan University (Natural Science Edition)
关键字：乳腺癌; 耐药性; 阿霉素; CdTe量子点; 增敏; 自噬
Key words：Breast cancer; Drug resistance; Doxorubicin; CdTe quantum dots; Sensitizing; Autophagy
基金项目：国家自然科学基金(31100716, U1332119)
中文摘要
利用纳米材料CdTe量子点作为增敏剂, 四甲基偶氮唑蓝(MTT)法检测细胞活力的实验表明CdTe量子点增强了阿霉素对于MCF 7/ADM的毒性, 而对非耐药的MCF 7无增敏作用. 然后利用免疫印迹法和免疫荧光染色法检测发现CdTe量子点提高了MCF 7/ADM细胞的自噬水平, 且这种自噬水平的上调与化学增敏效应是相关的, 以上结果提示CdTe量子点通过诱导MCF 7/ADM细胞发生自噬, 增强耐药性细胞对DOX毒性的敏感性.
英文摘要
CdTe quantum dots (QDs) were utilized as a sensitizer, Cell viability via MTT assay indicated that CdTe QDs enhanced the sensitivity to DOX for MCF 7/ADM cells but not for non resistant MCF 7 cells. Further studies using immunoblotting and immunofluorescence microscopy revealed that CdTe QDs up regulated autophagy in MCF 7/ADM cells, and increased level of autophagy are related to their ability of chemosensitization. These results suggest that autophagy is part of the machanism for CdTe QDs induced chemosensitization.
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