四川省结核分枝杆菌基因分型中MIRU VNTR 位点稳定性评价及保守位点分析
Analysis and evaluation of the stability and conservation of MIRU VNTR loci of Mycobacterium tuberculosis in Sichuan
作者：罗 槑(四川大学生命科学学院)；郑 超(四川大学生命科学学院)；吴桂辉(成都市公共卫生临床医疗中心)；李 松(四川大学生命科学学院)；孙 群(四川大学生命科学学院)；徐园红(成都市公共卫生临床医疗中心)；韩源平(四川大学生命科学学院)；岳 冀(成都市公共卫生临床医疗中心)
Author：LUO Mei(College of Life Sciences, Sichuan University)；ZHENG Chao(College of Life Sciences, Sichuan University)；WU Gui Hui(Chengdu Public Health Clinical Center)；LI Song(College of Life Sciences, Sichuan University)；SUN Qun(College of Life Sciences, Sichuan University)；XU Yuan Hong(Chengdu Public Health Clinical Center)；HAN Yuan Ping(College of Life Sciences, Sichuan University)；YUE Ji(Chengdu Public Health Clinical Center)
收稿日期：2015-01-28 年卷（期）页码：2015,52(5):1182-1186
期刊名称：四川大学学报: 自然科学版
Journal Name：Journal of Sichuan University (Natural Science Edition)
关键字：结核分枝杆菌；数目可变串联重复序列多态性；可变数目串联重复国际类型；四川地区
Key words：Mycobacterium tuberculosis; MIRU VNTR; MIT; Sichuan
基金项目：四川省卫生厅科研课题（090014）；成都市卫生局科学研究青年基金（0911）
中文摘要
本文收集了四川省2009～2013年184株结核分枝杆菌菌株，用标准12 MIRU-VNTR位点组对四川省结核分枝杆菌菌株进行基因分型研究，评估四川地区一线VNTR分型位点的稳定性，确定四川地区保守位点组合及主要MIT型.184株结核分枝杆菌基因分型后得到51个基因型，12个基因型成簇.本研究确定当前四川地区12高分辨力位点VNTR分型方法中的7个MIRU位点依然具有较高的分辩能力（h>055）.MIRU02, MIRU20, MIRU23, MIRU24为多态性最低的4个位点（0 ≤h
英文摘要
The standard 12 locus mycobacterial interspersed repetitive units variable number tandem repeats (MIRU VNTR) was performed on 184 M. tuberculosis isolates which were randomly selected from the collection during 2009 to 2013 in Sichuan province to confirm the set of conservative loci and the predominant MIT of the set of poorest discriminative loci as well as to evaluate the stability of the set of the first line typing loci using in this region. The study showed 184 M. tuberculosis isolates were divided into 51 genotypes and 12 clusters. We confirmed that the 7 MIRU loci which were included in the adjusted scheme of the 12 highly discriminatory VNTR loci were effective (h>055) and the 4 poorest discriminative loci were MIRU02, MIRU20, MIRU23, MIRU24 (0≤h
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