BK病毒样颗粒制备及血清学检测方法的建立
Preparation of virus like particles and establishment of the serological detection method for BK polyomavirus in China
作者：李晓雨(四川大学生命科学学院)；刘瑞熙(成都蓉生药业有限责任公司)；杨春(成都蓉生药业有限责任公司)；李小姣(成都蓉生药业有限责任公司)；唐诗佳(成都蓉生药业有限责任公司)；杨硕(成都蓉生药业有限责任公司)；武志强(成都蓉生药业有限责任公司)；刘兰军(成都蓉生药业有限责任公司)；鲍锦库(四川大学生命科学学院)
Author：LI Xiao Yu(College of Life Sciences, Sichuan University)；LIU Rui Xi(Chengdu Rongsheng Pharmaceuticals, Co., Ltd)；YANG Chun(Chengdu Rongsheng Pharmaceuticals, Co., Ltd)；LI Xiao Jiao(Chengdu Rongsheng Pharmaceuticals, Co., Ltd)；TANG Shi Jia(Chengdu Rongsheng Pharmaceuticals, Co., Ltd)；YANG Shuo(Chengdu Rongsheng Pharmaceuticals, Co., Ltd)；WU Zhi Qiang(Chengdu Rongsheng Pharmaceuticals, Co., Ltd)；LIU Lan Jun(Chengdu Rongsheng Pharmaceuticals, Co., Ltd)；BAO Jin Ku(College of Life Sciences, Sichuan University)
收稿日期：2014-08-28 年卷（期）页码：2015,52(6):1404-1410
期刊名称：四川大学学报: 自然科学版
Journal Name：Journal of Sichuan University (Natural Science Edition)
关键字：BK病毒; 病毒样颗粒; 血清学检测; 间接酶联免疫吸附试验
Key words：BK polyomavirus; Virus like particles; Serological detection; Indirect enzyme linked immunosorbent assay
基金项目：国家自然科学基金（81373311,31300674,81173093,30970643，J1103518）; 四川省青年科技创新研究团队（2011JTD0026）
中文摘要
将BK病毒 ( BK polyomavirus, BKV )的结构蛋白VP1 通过重组杆状病毒在昆虫细胞中表达, 并自发装配成BKV病毒样颗粒(BK virus like particles, BK VLPs). 以BK VLPs作为包被抗原建立了间接酶联免疫吸附试验(enzyme linked immunosorbent assay, ELISA)的血清学检测方法: 抗原最佳包被浓度为3.1 μg/mL, 血清最佳稀释度为1∶200, 酶标抗体最佳工作稀释度为1∶40000.并以该方法在国内对540名健康成年人进行BKV抗体阳性率情况调查, 结果显示我国健康成年人BKV抗体阳性率为79.94%.
英文摘要
In this study, the expression of viral capsid proteinVP1 of BK polyomavirus (BKV) in insect cells was reported. The expressed BKV VP1s were demonstrated to be self assembled into BKV virus like particles (BK VLPs) and could be used as antigen to establish the indirect enzyme linked immunosorbent assay (ELISA) for detection of human serum antibody to BK virus. The optimal concentration of coated antigen was 3.1μg/mL, and the best dilution of human serum samples and HRP labeled secondary antibody was 1∶200 and 1∶40000, respectively. Total 540 serum samples of healthy Chinese adults were investigated in their serum antibodies to BKV by using the established method and the results showed that the BKV antibody positive rate of the healthy adults in China was 79.94%.
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