镍矿区紫茎泽兰根际及内生细菌多样性研究
Diversity of rhizospheric and entophytic bacteria of Eupatorium adenophorum in nickel mine
作者：陈诚(四川农业大学资源环境学院微生物系)；赵珂(四川农业大学资源环境学院微生物系)；李小林(四川省农业科学院土壤肥料研究所)；张波(四川农业大学资源环境学院微生物系)；辜运富(四川农业大学资源环境学院微生物系)；郑林用(四川省农业科学院)；张小平(四川农业大学资源环境学院微生物系)
Author：CHEN Cheng(Department of Microbiology, College of Resource and Environment, Sichuan Agricultural University)；ZHAO Ke(Department of Microbiology, College of Resource and Environment, Sichuan Agricultural University)；LI Xiao Lin(Soil and Fertilizer Institute, Sichuan Academy of Agricultural Sciences,)；ZHANG Bo(Department of Microbiology, College of Resource and Environment, Sichuan Agricultural University)；GU Yun Fu(Department of Microbiology, College of Resource and Environment, Sichuan Agricultural University)；ZHENG Lin Yong(Sichuan Academy of Agricultural Sciences)；ZHANG Xiao Ping(Department of Microbiology, College of Resource and Environment, Sichuan Agricultural University)
收稿日期：2014-05-30 年卷（期）页码：2015,52(6):1387-1392
期刊名称：四川大学学报: 自然科学版
Journal Name：Journal of Sichuan University (Natural Science Edition)
关键字：镍矿区; 紫茎泽兰; 细菌; 多样性; DGGE
Key words：Nickel mines; Eupatorium adenophorum; Bacteria; diversity; DGGE
基金项目：国家 863 计划(2013AA102802 05)
中文摘要
利用变性梯度凝胶电泳(denaturing gradient gel electrophoresis, DGGE)技术对四川省攀枝花市镍矿区紫茎泽兰根际及不同器官中内生细菌多样性进行分析. 结果表明, 紫茎泽兰根际与不同器官内生细菌多样性存在明显差异, 香农 威纳指数(H)、丰度(S)及均匀度(EH)皆表现为根际土＞叶＞茎＞根. 土壤重金属含量与内生细菌多样性相关性分析表明, 样品的丰度(S)与Zn含量呈显著正相关 (P
英文摘要
The denaturing gradient gel electrophoresis (DGGE ) method was applied to investigate the diversity of the rhizospheric bacteria and entophytic bacteria in different organs of Eupatorium adenophorum growing in Nickelin Nickel Mine of Panzhihua, Sichuan Province. The results indicated that the diversity of the rhizospheric and entophytic bacteria were significantly different. The highest diversity index (H) and richness (S) and evenness (EH) were found in rhizosphere sample, followed by leaf, stem and root. Correlation analysis indicated that the richness (S) showed significantly positive correlation with the concentrations of zinc (P
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