猪生长激素受体(GHR)基因启动子的
克隆及功能分析
Characterization of Growth Hormone Receptor (GHR) Gene Promoter in pigs: Molecular Cloning and Functional Analysis
作者：徐敏(四川大学生命科学学院生物资源与生态环境教育部重点实验室)；黄桂安(四川大学生命科学学院生物资源与生态环境教育部重点实验室)；孟风艳(四川大学生命科学学院生物资源与生态环境教育部重点实验室)；李娟(四川大学生命科学学院生物资源与生态环境教育部重点实验室)；王亚军(四川大学生命科学学院生物资源与生态环境教育部重点实验室)
Author：XU Min(Key laboratory of Bio resources and Eco environment of Ministry of Education,College of Life Sciences, Sichuan University)；HUANG Gui An(Key laboratory of Bio resources and Eco environment of Ministry of Education,College of Life Sciences, Sichuan University)；MENG Feng Yan(Key laboratory of Bio resources and Eco environment of Ministry of Education,College of Life Sciences, Sichuan University)；LI Juan(Key laboratory of Bio resources and Eco environment of Ministry of Education,College of Life Sciences, Sichuan University)；WANG Ya Jun(Key laboratory of Bio resources and Eco environment of Ministry of Education,College of Life Sciences, Sichuan University)
收稿日期：2013-04-11 年卷（期）页码：2015,52(6):1353-1358
期刊名称：四川大学学报: 自然科学版
Journal Name：Journal of Sichuan University (Natural Science Edition)
关键字：生长激素受体; 启动子; 活性分析拟南芥; 亚细胞定位; 热胁迫
Key words：Pig; Growth hormone receptor; Promoter; Functional analysis
基金项目：转基因生物新品种培育科技重大专项 (2009zx08009 145B)
中文摘要
本研究以猪作为研究模型, 对GHR基因启动子区开展了克隆 、转录因子结合位点分析和启动子活性鉴定.结果表明: 家猪GHR基因或由两个启动子(GHR P1和GHR P2)控制.GHR P1的部分核苷酸序列与牛、羊对应核苷酸序列具有较高的同源性, 但功能分析显示其启动子活性较低.针对GHR P2的实验结果表明其具有典型的GHR组成型启动子特征, 荧光素酶报告实验表明其具有启动子活性, 因此我们推测本次克隆获得的GHR P2是猪GHR的组成型启动子.
英文摘要
The putative promoter regions of GHR were cloned by using pig as model. Furthermore, the transcription factor binding site was analyzed by using online software and promoter activities in cultured HepG2 cells. The results showed that partial nucleotide sequences of GHR P1 shared high homology with liver specific promoters of bovine and ovine, but its promoter activity is not obvious. Meanwhile, GHR P2 possess classical features of GHR constitutive promoter and dual luciferase reporting assay showed that GHR P2 have obvious promoter activity, these results indicate that GHR P2 cloned in this study is a constitutive promoter in pig.
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