With the rapid development of molecular biology, the classify and identify of oral bacteria have come up to various molecule type classification standards from the traditional phenotype classification. Polyphasic taxonomy has synthesized the characteristics of phenotype, genotype and systematic growth, and it makes the classified method more objective and reasonable, representing the systematic evolution relationship between creatures. In this paper, several method in classify and identify of oral bacteria including electrophoresis, DNA G+C mole fraction, hybridization, the sequence analysis of 16S rRNA, metabonomics were reviewed.