溶血磷脂酸对牙髓细胞中β-连环蛋白核转位的影响及机制初探
The effects and underlying mechanism of lysophosphatidic acid in β-catenin nuclear translocation of dental pulp cells
作者：房宏志, 杨惠, 邵美瑛, 胡涛
Author：Fang Hongzhi, Yang Hui, Shao Meiying, Hu Tao
收稿日期：2017-05-15 年卷（期）页码：2018,45(1):26-31
期刊名称：国际口腔医学杂志
Journal Name：International Journal of Stomatology
关键字：牙髓细胞,溶血磷脂酸,Rho相关蛋白激酶,β-连环蛋白,
Key words：dental pulp cell,lysophosphatidic acid,Rho-associated protein kinase,β-catenin,
基金项目：
国家自然科学基金（81400504）; 四川省科学技术厅应用基础研究项目（2014JY0073,2013JY0164）; 中国博士后科学基金面上项目（2014M562332）
中文摘要
目的 采用体外培养的牙髓细胞,探讨溶血磷脂酸（LPA）对牙髓细胞内β-连环蛋白释放、活化及核转位的影响。方法 采用LPA刺激牙髓细胞,Y-27632阻断剂抑制Rho相关蛋白激酶（ROCK）,通过免疫荧光和Western blot检测Rho/ROCK信号通路对牙髓细胞β-连环蛋白释放、活化及核转位的影响。结果 LPA刺激牙髓细胞3 h,β-连环蛋白向核膜边缘集聚;刺激6、10 h,见部分牙髓细胞的β-连环蛋白转运至细胞核。用Y-27632预先阻断ROCK后,能够抑制LPA所引起的β-连环蛋白核内转位。Western blot检测结果显示,LPA促进β-连环蛋白表达及活化,Y-27632能够在一定程度上抑制LPA介导的β-连环蛋白的活化水平。结论 LPA能够通过Rho/ROCK通路影响牙髓细胞β-连环蛋白的释放、活化及核转位。
英文摘要
ObjectiveThe aim of our study is to investigate the biological effects of lysophosphatidic acid (LPA) on β-catenin accumulation, activation, and nuclear translocation of cultured human dental pulp cells (DPCs)ex vivo.MethodsLPA was used to stimulate dental pulp cells, and Y-27632 was used to inhibit Rho-associated protein kinase (ROCK). Fluorescence microscopy and Western blot were used to detect the effects of Rho/ROCK on β-catenin accumulation, activation, and nuclear transloca- tion.Resultsβ-catenin accumulated around the nuclear membrane after LPA stimulation of DPC for 3 h. When treated for 6 or 10 h with LPA, β-catenin translocated into the nucleus in some dental pulp cells; however, nuclear translocation of β-catenin was abrogated by Y-27632. Results of Western blot showed that LPA promoted β-catenin expression and activation. ROCK inhibition also degraded the level of β-catenin activation.ConclusionLPA regulated β-catenin accumulation, activation, and nuclear translocation via the Rho/ROCK signaling pathway.
上一条：接受计算机导航技术颌面部手术治疗患者的心理研究
下一条：改良介孔生物活性玻璃在颌面部骨缺损修复中的研究进展
【关闭】
