白藜芦醇诱导DT40细胞DNA损伤的机制研究
The mechanism of resveratrol induced DNA damages in DT40 cells
作者：胡晓青;刘颖;卿勇;
Author：
收稿日期： 年卷（期）页码：2018,33(01):-31-33
期刊名称：华西药学杂志
Journal Name：WEST CHINA JOURNAL OF PHARMACEUTICAL SCIENCES
关键字：白藜芦醇;DT40细胞;活性氧;抗氧化剂;DNA损伤;MTT法;染色体畸变分析
Key words：
基金项目：
中文摘要
目的研究白藜芦醇(RSV)诱导DT40细胞DNA损伤的作用机制。方法采用MTT试验检测RSV对细胞增殖的影响;利用染色体畸变试验分析RSV对细胞的DNA损伤作用以及抗氧剂N-乙酰半胱氨酸(NAC)对RSV诱导DNA损伤的干扰作用。结果 50μmol·L~(-1)RSV连续处理16 h后可诱导DT40细胞的DNA损伤;RSV对DT40细胞的增殖呈剂量依赖性抑制作用,1 mmol·L~(-1)NAC预处理1 h后可提高DT40细胞的生存率,同时也可抑制RSV诱导的DNA损伤。结论 RSV可诱导DT40细胞的DNA损伤,其作用机制可能与RSV的氧化损伤相关。
参考文献
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