伏康树籽中吲哚类生物碱的分离与纯化
Separation and purification of the indole alkaloids from seeds of Voacanga africana
作者：李琴;白珂珂;李延芳;
Author：
收稿日期： 年卷（期）页码：2018,33(01):-16-19
期刊名称：华西药学杂志
Journal Name：WEST CHINA JOURNAL OF PHARMACEUTICAL SCIENCES
关键字：伏康树籽;吲哚类生物碱;半制备高效液相色谱法;分离纯化;结构鉴定
Key words：
基金项目：
中文摘要
目的分离并纯化伏康树籽中的吲哚类生物碱。方法先采用传统的柱层析法分离其醇提物,再采用半制备HPLC系统进行纯化,收集对应峰的流出液,检测产品的纯度,结合理化常数测定及波谱学技术确定其结构。结果通过传统柱层析法分离得到生物碱类化合物柳叶水甘草碱(Ⅳ),经过半制备液相色谱法分离得到3-oxotabersoine(Ⅰ)、(-)-pachysiphine(Ⅱ)、(-)-lochnericine(Ⅲ)等生物碱单体。结论除化合物Ⅳ外,其余3个化合物均为首次从该属植物中分离得到。利用半制备色谱技术和传统柱层析法结合,分离纯化伏康树籽中结构类似的吲哚生物碱,具有简便、快捷和周期短等优点。
参考文献
[1]Koroch AR,Juliani HR,Kulakowski D,et al.Voacanga africana:chemistry,quality and pharmacological activity[M].Washington,DC:American Chemical Society,2009:363-380.
[2]Tona L,Kambu K,Ngimbi N,et al.Antiamoebic and phytochemical screening of some Congolese medicinal plants[J].J Ethnopharm,1998,65(1):57-65.
[3]苏艳红,陈晓春,白珂珂,等.伏康树籽挥发油的GC-MS分析[J].药物分析,2011,31(3):496-498.
[4]白珂珂,苏艳红,陈晓春,等.HPLC测定伏康树籽中的柳叶水甘草碱[J].华西药学杂志,2011,26(5):492-493.
[5]梅玲,邓赟,李甫,等.伏康树中生物碱化学成分研究[J].中草药,2012,32(2):226-229.
[6]Aimi N,Asada Y,Saka SI,et al.Studies on plants containing indole alkaloids.Ⅶ.isolation of several aspidosperma-and vincamine-type alkaloids from the seeds of Amsonia elliptical[J].Chem Pharm Bull,1978,26(4):1182-1187.
[7]Rolland Y,Kunesch N,Poisson J,et al.Carbon-13 nuclear magnetic resonance spectroscopy of naturally occurring substances.43.Carbon-13 nuclear magnetic resonance analysis of bisindoline alkaloids of two voacanga species[J].J Org Chem,1976,41(20):3270-3275.
[8]Eles J,Kalaus G,Greiner I,et al.Synthesis of vinca alkaloids and related compounds.100 stereoselective oxidation reactions of compounds with the aspidospennane and quebrachamine ring system.First synthesis of some alkaloids containing the epoxy ring[J].J Org Chem,2002,67(21):7255-7260.
【关闭】
