白蛋白亲和肽-黑色素瘤抗原肽胶束淋巴结的靶向性研究
Study on targeting lymph nodes of the micelle from albumin binding domain and melanoma antigen peptide
作者：李琳;张志荣;龚涛;孙逊;
Author：
收稿日期： 年卷（期）页码：2018,33(01):-1-4
期刊名称：华西药学杂志
Journal Name：WEST CHINA JOURNAL OF PHARMACEUTICAL SCIENCES
关键字：淋巴结靶向;白蛋白;白蛋白亲和肽;黑色素瘤抗原肽;自组装;胶束;亲和性;多肽;疫苗
Key words：
基金项目：国家自然科学基金资助项目(批准号:81690261、81673362)
中文摘要
目的制备环状白蛋白亲和肽-黑色素瘤抗原肽(cABD-Trp2)胶束,探究胶束与白蛋白的亲和力及其淋巴结的靶向性。方法具白蛋白亲和性的环状多肽序列与抗原肽共价连接后,在水溶液中自组装成cABD-Trp2胶束,利用透射电镜观察其形态和粒径;通过非变性聚丙烯酰胺凝胶电泳、荧光能量共振转移、生物膜层干涉技术来考察胶束与白蛋白间的亲和性;经荧光成像系统来考察cABD-Trp2胶束在体内对淋巴结的靶向性。结果成功制备了cABD-Trp2胶束,其形态为不规则的球形,粒径约25 nm。胶束形成后仍能与白蛋白亲和,与游离抗原或弗氏不完全佐剂载抗原比较,胶束能显著提高抗原对淋巴结的靶向性。结论白蛋白亲和多肽与抗原肽形成胶束后仍能与白蛋白有效亲和,通过内源性白蛋白,可使抗原有效传递到淋巴结,为疫苗设计提供了新思路。
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