注射剂微生物污染的RiboPrinter~?微生物基因指纹系统鉴定及同源性分析
Identification and homology analysis of microbial contamination in injection with RiboPrinter~? system
作者：孙雪奇;秦力;陈婕;费国琴;袁军;
Author：
收稿日期： 年卷（期）页码：2019,34(05):-481-484
期刊名称：华西药学杂志
Journal Name：WEST CHINA JOURNAL OF PHARMACEUTICAL SCIENCES
关键字：微生物污染;鉴定;同源性分析;葡萄球菌;RiboPrinter~?微生物基因指纹系统;生化鉴定;阳性结果;无菌检查;相似系数
Key words：
基金项目：四川省成都市科技局科技惠民技术研发项目(2016-HM01-00087-SF)
中文摘要
目的对注射剂的无菌检查阳性结果进行核糖体分型鉴定,并根据鉴定结果进行微生物污染的来源分析。方法采用RiboPrinter~?微生物基因指纹系统对阳性菌A1和污染调查中采集菌株中的的16株葡萄球菌进行鉴定和同源性分析。结果 RiboPrinter~?微生物基因指纹系统的鉴定结果与菌株的生化鉴定结果完全相同; A1的鉴定结果为科氏葡萄球菌,溯源自无菌检查实验的环境污染。结论基于核糖体分型原理的RiboPrinter~?微生物基因指纹系统鉴定结果准确可靠,可用于鉴定及同源性分析污染的微生物。
参考文献
[1]中华人民共和国卫生部．药品生产质量管理规范(2010修订)[S]. 2010.
[2]陈伟盛，关倩明，朱荣峰，等．药品微生物限度检查中微生物污染的鉴定和溯源分析[J]．药物分析杂志，2014,34(1):58-63.
[3] FC Tenover,RD Arbeit,RV Goering,et al. Interpreting chromosomal DNA restriction patterns produced by pulsed-field gel electrophoresis:Criteria for bacterial strain typing[J]. J Clin Microbiol,1995,33:2233-2239.
[4] Valerie B,Heather H,Richard G. Molecular Genetic Basis of Ribotyping[J]. Clin Microbiol Rev,2008:262-273.
[5] Aarestrup FM. Comparative ribotyping of Staphylococcus intermedius isolated from members of the Canoidea gives possible evidence for hostspecificity and co-evolution of bacteria and hosts[J]. J Syst Evol Microbiol,2001,51:1343-1347.
[6] Pitt TL,Trakulsomboon S,Dance DA. Molecular phylogeny of Burkholderia pseudomallei[J]. Acta Trop,2000,74:181-185.
[7]刘秀梅，周正，郭云昌．食源性沙门氏菌分离株自动化核糖体分型的研究[J]．中国食品学报，2006,6(2):1-5.
[8] M Lipsitch,K O’Neill,D Cordy,et al. Strain characteristics of Streptococcus pneumoniae carriage and invasive disease isolates during a cluster-randomized clinical trial of the 7-valent pneumococcal conjugate vaccines[J]. J Infect Dis,2007,196:1221-1227.
[9] Connie SP,Holly H,Suzanne P,et al. Comparison of an automated ribotyping system to restriction endonuclease analysis and pulsed-field gel electrophoresis for differentiating vancomycin-resistant enterococcus faecium isolates[J]. J Clin Microbiol,2002,40(5):1858-1861.
【关闭】
