软刀素肽的固相合成
Solid-phase synthesis of urumin
作者：莫金秋;刘泰容;苏春丽;廖洪利;胡宏岗;
Author：
收稿日期： 年卷（期）页码：2018,33(04):-356-358
期刊名称：华西药学杂志
Journal Name：WEST CHINA JOURNAL OF PHARMACEUTICAL SCIENCES
关键字：软刀素肽;H1型病毒;固相合成;高效液相色谱法;高分辨质谱;多肽;王树脂;1-羟基苯并三唑/N,N-二异丙基碳二亚胺
Key words：
基金项目：四川省教育厅科研基金重点项目(18ZA0149)
中文摘要
目的制备抗H1型病毒的软刀素肽。方法采用9-芴甲氧羰基(Fmoc)固相合成法,选用Wang树脂为载体,以HOBt/DIC为缩合试剂依次偶联氨基酸后,将多肽从树脂上切割下来,得到的粗品采用制备HPLC进行纯化。结果制得纯度>98.2%的多肽产物,用HPLC归一化法测定的含量为26.3%,所得产物通过HRMS分析,确定为软刀素肽。结论所用方法可行性高、操作简便,收率较高,目标化合物可用于抗H1型病毒的后续药物研究。
参考文献
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