环巴胺固体脂质纳米粒的研究
Study on cyclopamine-loaded solid lipid nanoparticles
作者：丁倩;李寒梅;孙逊;
Author：
收稿日期： 年卷（期）页码：2017,32(01):-9-12
期刊名称：华西药学杂志
Journal Name：WEST CHINA JOURNAL OF PHARMACEUTICAL SCIENCES
关键字：固体脂质纳米粒;高效液相色谱法;环巴胺;Hedgehog信号通路;RT-PCR
Key words：
基金项目：
中文摘要
目的制备环巴胺固体脂质纳米粒(Cyc-SLNs),并考察其理化性质和对Hedgehog信号通路的抑制作用。方法采用薄膜超声法制备Cyc-SLNs,并考察其对B16F10黑色素瘤细胞增殖、细胞周期、凋亡和Hedgehog信号通路靶基因Gli1表达的影响。结果制得的Cyc-SLNs呈平整的球形,平均粒径、多分散指数(PDI)、Zeta电位和平均包封率分别为126.5±2.1 nm、0.198±0.007、23.5±0.7 m V、91.59%±0.37%;Cyc-SLNs对B16F10细胞增殖分裂的抑制、凋亡的促进和Gli1表达的下调均强于环巴胺。结论成功制备了抗肿瘤细胞效果强于环巴胺的固体脂质纳米粒Cyc-SLNs。
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