不同细胞外泌体的分离与特性研究
Isolation and characterization of different extracellular exosomes
作者：李昕;刘瑜;尹宗宁;
Author：
收稿日期： 年卷（期）页码：2018,33(06):-480-483
期刊名称：华西药学杂志
Journal Name：WEST CHINA JOURNAL OF PHARMACEUTICAL SCIENCES
关键字：外泌体;A549细胞;B16F10细胞;表征;分离;鉴定;促增殖
Key words：
基金项目：
中文摘要
目的探究人肺癌细胞A549与小鼠黑色素瘤细胞B16-F10不同细胞外泌体的性质及促细胞增殖能力的差异。方法采用超速离心法及Exo Qμick试剂盒法提取A549细胞及B16-F10细胞培养上清液中的外泌体;利用透射电镜观察外泌体的形态,采用动态光散射法测定粒径,用SDS-PAGE电泳技术分析外泌体的蛋白分布,采用Western blot技术分析其特异蛋白TSG 101的表达,用MTT法测定外泌体促细胞的增殖能力。结果超速离心法和Exo Qμick试剂盒法均可用于提取外泌体,A549及B16-F10细胞外泌体均呈圆形膜性囊泡结构;提取的外泌体样本均有外泌体特异性标记蛋白TSG 101的表达,B16-F10细胞外泌体促细胞增殖能力更佳。结论超速离心法分离的外泌体纯度高,促细胞增殖能力好。
参考文献
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