青蒿琥酯抑制Twist 1蛋白表达和血管再生以及降低口腔鳞癌细胞Tca8113转移的作用
Study of the function of artemisinin on inhibiting the expression of Twist 1 proteins and angiogenesis and decreasing the transfer of human oral squamous carcinoma Tca8113 cells
作者：吴晓洪;郝玉梅;
Author：
收稿日期： 年卷（期）页码：2017,32(02):-172-175
期刊名称：华西药学杂志
Journal Name：WEST CHINA JOURNAL OF PHARMACEUTICAL SCIENCES
关键字：青蒿琥酯;Twist 1蛋白;口腔鳞癌细胞;增殖;迁移;凋亡;血管再生
Key words：
基金项目：
中文摘要
目的研究青蒿琥酯抑制Twist 1表达、降低口腔鳞癌细胞的转移及对血管生成的抑制作用。方法采用50、100、150、200μg·m L~(-1)的青蒿琥酯体外培养的口腔鳞癌细胞,然后分别采用Western blot法、MTT法、划痕实验及流式细胞仪检测不同浓度的青蒿琥酯干预对口腔鳞癌细胞Twist1表达、增殖、迁移及凋亡的影响;采用鸡胚尿囊膜检测青蒿琥酯对鸡胚尿囊膜血管生成的影响。结果与对照组对比,青蒿琥酯可抑制Twist1蛋白的表达、口腔鳞癌细胞的增殖和迁移,且存在时间和剂量依赖性;青蒿琥酯可引起细胞的凋亡,处理组的细胞凋亡率显著高于对照组;青蒿琥酯对鸡胚尿囊膜血管新生有一定的抑制作用(P
参考文献
[1]米热古丽·阿不都热合曼,杜探·赛肯,刘丹玉.吉西他滨联合顺铂一线治疗对Ⅲb~Ⅳ期肺鳞癌的疗效评价[J].实用肿瘤杂志,2015,30(5):459-462.
[2]Li P C,Lam E,Roos W P,et al.Artesunate derived from traditional Chinese medicine induces DNA damage and repair[J].Cancer Res,2008,68(11):4347-4351.
[3]Efferth T,Dunstan H,Sauerbrey A,et al.The anti-malarial artesunate is also active against cancer[J].Inter J Oncology,2001,18(4):767-773.
[4]Ma H,Yao Q,Zhang A M,et al.The effects of artesunate on the expression of EGFR and ABCG2 in A549 human lung cancer cells and a xenograft model[J].Molecules,2011,16(12):10556-10569.
[5]Tan W,Lu J,Huang M,et al.Anti-cancer natural products isolated from Chinese medicinal herbs[J].Chinese Medicine,2011,6(1):1-15.
[6]Mao Z G,Zhou J,Wang H,et al.Artesunate inhibits cell proliferation and decreases growth hormone synthesis and secretion in GH3 cells[J].Molecular Biology Reports,2012,39(5):6227-6234.
[7]Yang J,Mani SA,Donaher JL,et al.Twist,a master regulator of morphogenesis,plays an essential role in tumor metastasis[J].Cell,2004,117(7):927-39.
[8]Li Y,Shan F,Wu JM,et al.Novel antitumor artemisinin derivatives targeting G1 phase of the cell cycle[J].Chem Inform,2001,32(18):5-8.
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