金荞麦提取物改善肠易激综合征大鼠内脏痛觉过敏的效果及其机制
Effects of Fagopyrum cymosum extracts on visceral hyperalgesia of IBS rats and the mechanism
作者：刘丽娜;严晶;陆玮婷;陈广梅;陆茵;孙志广;
Author：
收稿日期： 年卷（期）页码：2016,31(02):-135-138
期刊名称：华西药学杂志
Journal Name：WEST CHINA JOURNAL OF PHARMACEUTICAL SCIENCES
关键字：金荞麦提取物;肠易激综合征;内脏痛觉过敏;腹壁撤退反射;脊髓背根神经节;TRPV1受体;辣椒素
Key words：
基金项目：国家自然科学基金青年科学基金资助项目(批准号:81503536);;
江苏省自然科学基金青年基金资助项目(BK20140959;BK20151008);;
江苏省高校自然科学研究面上项目(No.15KJB360008);;
高等学校博士学科点专项科研基金联合资助课题(No.20133237110008)
中文摘要
目的观察金荞麦提取物(Fag)对肠易激综合征(IBS)大鼠内脏痛觉过敏的影响及其机制。方法采用腹壁撤退反射(AWR)评分观察Fag(6、24 g·kg-1)口服给药两周后的IBS大鼠模型的内脏痛觉过敏的变化,并用免疫荧光法观察腰骶段脊髓背根神经(DRG)的辣椒素受体亚型TRPV1表达水平;采用细胞膜片钳记录Fag(0.1、0.3、1、3 g·L-1)对辣椒素(CAP)激活大鼠的DRG细胞TRPV1电流(ITRPV1)的影响。结果 Fag 24 g·kg-1在体内可显著降低升高的IBS大鼠AWR评分,IBS大鼠腰骶段DRG的TRPV1表达增高;Fag 6、24 g·kg-1干预后DRG的TRPV1表达下降;体外0.1、0.3、1、3 g·L-1Fag可浓度依赖地抑制CAP激活的分离DRG神经元ITRPV1,IC50为0.83 g·L-1。结论 Fag可通过下调IBS大鼠TRPV1受体表达改善大鼠痛觉过敏,可抑制DRG神经元ITRPV1峰值,调节痛觉信号传递。
参考文献
[1]Nozu T,Kudaira M,Kitamori S,et al.Repetitive rectal painful distention induces rectal hypersensitivity in patients with irritable bowel syndrome[J].J Gastroenterol,2006,41(3):217-222.
[2]Chan CL,Facer P,Davis JB,et al.Sensory fibres expressing capsaicin receptor TRPV1 in patients with rectal hypersensitivity and faecal urgency[J].Lancet,2003,361(9355):385-391.
[3]Oh U,Hwang SW,Kim D.Capsaicin activates a nonselective cation channel in cultured neonatal rat dorsal root ganglion neurons[J].J Neurosci,1996,16(5):1659-1667.
[4]Dhaka A,Uzzell V,Dubin AE,et al.TRPV1 is activated by both acidic and basic p H[J].J Neurosci,2009,29(1):153-158.
[5]Calixto JB,Kassuya CA,Andre E,et al.Contribution of natural products to the discovery of the transient receptor potential(TRP)channels family and their functions[J].Pharmacol Ther,2005,106(2):179-208.
[6]孙志广,周云中,王兆龙.金荞麦胶囊、片在制备治疗肠易激综合症的药物中的应用[P].CN:200710022046.3.
[7]Al-Chaer ED,Kawasaki M,Pasricha PJ.A new model of chronic visceral hypersensitivity in adult rats induced by colon irritation during postnatal development[J].Gastroenterology,2000,119(5):1276-1285.
[8]Gerke MB,Plenderleith MB.Ultrastructural analysis of the central terminals of primary sensory neurones labelled by transganglionic transport of bandeiraea simplicifolia I-isolectin B4[J].Neuroscience,2004,127(1):165-175.
[9]Chadwick VS,Chen W,Shu D,et al.Activation of the mucosal immune system in irritable bowel syndrome[J].Gastroenterology,2002,122(7):1778-1783.
[10]Dal Bo S,Jurgensen S,Horst H,et al.Antinociceptive effect of proanthocyanidins from Croton celtidifolius bark[J].J Pharm Pharmacol,2005,57(6):765-771.
【关闭】
