盐酸胍诱导辣根过氧化物酶变性过程中酶构象-活力研究
Study on the conformation-activity relationship during the denaturation process of horseradish peroxidase induced by guanidine hydrochloride
作者：张家兴;李小丝;尹宗宁;
Author：
收稿日期： 年卷（期）页码：2016,31(03):-273-277
期刊名称：华西药学杂志
Journal Name：WEST CHINA JOURNAL OF PHARMACEUTICAL SCIENCES
关键字：辣根过氧化物酶;盐酸胍;酶变性;模型;可见光光谱法;荧光光谱法;荧光相图法;构象-活力关系
Key words：
基金项目：国家自然科学基金资助项目(批准号:81373338)
中文摘要
目的研究辣根过氧化物酶(HRP)在盐酸胍中的变性过程,并建立荧光参数与酶活力的函数模型。方法采用可见光谱法,荧光光谱法及荧光相图法对盐酸胍诱导的HRP变性过程进行研究,并采用SPSS和Origin软件对变性过程中的构象参数与酶活力参数进行函数模型拟合。结果盐酸胍诱导的HRP去折叠经历了一个三态过程,其中,引起酶分子从部分折叠中间态向去折叠态转变,导致其疏水内核构象剧烈变化,酶活性中心结构及酶活力开始变化的临界盐酸胍浓度为1.5 mol·L-1;同时建立了构象参数与酶活力的函数模型。结论所建模型可以表征HRP的变性过程并用于酶活力的预测。
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