冬凌草甲素逆转SGC7901/ADR细胞的多药耐药性及其机制
Reversal effect of oridonin on multidrug resistance cells SGC7901/ADR and its mechanism
作者：许文婷;高巧慧;伏艳艳;吴臻;李发荣;
Author：
收稿日期： 年卷（期）页码：2016,31(03):-262-264
期刊名称：华西药学杂志
Journal Name：WEST CHINA JOURNAL OF PHARMACEUTICAL SCIENCES
关键字：冬凌草甲素;P-糖蛋白;多药耐药性;阿霉素;MDR1基因;SGC7901/ADR细胞;机制;表达
Key words：
基金项目：陕西省自然科学基金项目(2012JM4010);;
中央高校基本科研业务费专项基金项目(GK261001001)
中文摘要
目的观察冬凌草甲素(Ori)对胃癌细胞株SGC7901/ADR多药耐药性的逆转及其作用机制。方法用MTT法检测Ori的非细胞毒浓度及对SGC7901/ADR细胞株阿霉素敏感性的影响;免疫荧光法检测Ori对SGC7901/ADR细胞P-糖蛋白(P-gp)活性及表达的影响;RT-PCR法检测Ori对细胞MDR1基因转录的影响。结果用Ori无毒剂量处理细胞后,细胞对阿霉素的逆转倍数为4.53,MDR1基因的转录降低了0.78,P-gp的表达率降低了18.83%。结论 Ori能逆转SGC7901/ADR细胞的多药耐药性,其机制为降低MDR1基因的转录和下调P-gp的表达。
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