IL-10免疫黏附素和肿瘤相关巨噬细胞对卵巢癌侵袭性的研究
Study on immunoadhesins for IL-10 and tumor associated macrophages on the invasion of ovarian cancer
作者：李乔;尹如铁;周乐;高倩;牛艺臻;
Author：
收稿日期： 年卷（期）页码：2017,32(03):-257-259
期刊名称：华西药学杂志
Journal Name：WEST CHINA JOURNAL OF PHARMACEUTICAL SCIENCES
关键字：IL-10免疫黏附素;白细胞介素10拮抗剂;白细胞介素10;肿瘤相关巨噬细胞;卵巢癌;缺氧诱导因子-1α;肿瘤微环境;侵袭性
Key words：
基金项目：中国癌症基金会资助项目(批准号:S2014025)
中文摘要
目的检测IL-10免疫黏附素和肿瘤相关巨噬细胞(TAM)对卵巢癌细胞侵袭性的影响,并研究其关系。方法通过刺激人急性单核白血病细胞(THP-1)分化为巨噬细胞,模拟体内的TAM,进行培养,取该培养基作为TAM条件培养基,并用其培养人卵巢癌细胞株ES-2细胞;采用Matrigel invasion assay观察穿过transwell小室的细胞数目,确定TAM对ES-2细胞侵袭性的影响;采用Western blot法检测TAM培养基处理后的ES-2细胞中缺氧诱导因子1α(HIF-1α)蛋白水平的变化。并通过IL-10免疫黏附素观察其对ES-2细胞HIF-1α蛋白水平及侵袭性的影响。结果加入TAM条件培养基的ES-2细胞的侵袭性明显高于正常培养基组(P
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