丹参酮类化合物诱导白血病干细胞凋亡的体外研究
In vitro study of leukemia stem cells apoptosis induced by tanshinone compounds
作者：李慧;向洪;王银;卿红;王春森;
Author：
收稿日期： 年卷（期）页码：2016,31(04):-356-358
期刊名称：华西药学杂志
Journal Name：WEST CHINA JOURNAL OF PHARMACEUTICAL SCIENCES
关键字：二氢丹参酮Ⅰ;丹参酮Ⅰ;隐丹参酮;丹参酮ⅡA;细胞凋亡;白血病干细胞;CD123+;NB4;Annexin V/PI双标记法
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基金项目：
中文摘要
目的探讨丹参酮类化合物对白血病干细胞(LSCs)的促凋亡作用。方法将二氢丹参酮Ⅰ、丹参酮Ⅰ、隐丹参酮与丹参酮ⅡA等4种丹参酮类化合物分别作用于白血病NB4细胞,以CD34~+、CD38~-、CD123~+为标记确定NB4细胞中的LSCs,采用Annexin V/PI双标记法检测LSCs的凋亡率。结果在20μmol·L~(-1)二氢丹参酮Ⅰ和丹参酮Ⅰ条件下作用24 h后,可显著诱导LSCs凋亡,而隐丹参酮和丹参酮ⅡA不能诱导LSCs凋亡。结论丹参酮类化合物诱导NB4中LSCs的凋亡能力与其结构有关,与课题组前期报道的其对NB4细胞的增殖抑制作用基本一致。
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