微针促渗仪经皮免疫部位对免疫应答的影响
Effects of microneedle-pretreated immune sites on immune responses
作者：彭珂;郑涛;张志荣;孙逊;
Author：
收稿日期： 年卷（期）页码：2017,32(05):-518-520
期刊名称：华西药学杂志
Journal Name：WEST CHINA JOURNAL OF PHARMACEUTICAL SCIENCES
关键字：微针促渗;穿刺能力;经皮免疫;免疫应答;耳部给药;抗原;佐剂;抗体
Key words：
基金项目：
中文摘要
目的利用微针的促渗作用对小鼠进行经皮免疫,探讨不同免疫部位所引起免疫应答的特点。方法用染色法和组织切片法考察微针促渗仪的穿刺能力和穿刺效果;采用微针促渗仪,对小鼠的后背皮肤和耳部皮肤进行处理,涂抹模型抗原卵清蛋白(OVA)和佐剂Cp G ODN 1826后,采用ELISA法测定血清中的抗OVA特异性抗体。结果微针促渗仪可成功穿刺皮肤角质层,将抗原OVA和佐剂Cp G ODN 1826同时递送至皮肤表皮层。与经后背皮肤免疫比较,经耳部免疫会产生更强效的Ig G、Ig G 1和Ig G 2a特异性抗体水平。结论经皮免疫在耳部皮肤引起了比后背皮肤更强的免疫效果。
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