聚苯乙烯纳米粒对细胞活性氧的影响
Effect of polystyrene nanoparticles on HK-2 intracellular ROS
作者：谭灵莉;宋旭;张志荣;
Author：
收稿日期： 年卷（期）页码：2017,32(05):-493-494
期刊名称：华西药学杂志
Journal Name：WEST CHINA JOURNAL OF PHARMACEUTICAL SCIENCES
关键字：活性氧;纳米粒;HK-2细胞;聚苯乙烯;DCFH-DA
Key words：
基金项目：
中文摘要
目的观察聚苯乙烯纳米粒对人肾小管上皮细胞(HK-2)内活性氧水平的影响。方法用不同浓度的PS、氨基聚苯乙烯(PS-NH2)及羧基聚苯乙烯(PS-COOH)纳米粒刺激HK-2细胞0.5 h,经DCFH-DA染色后,用流式细胞仪检测其平均荧光强度。结果 0.1μg·m L~(-1)PS纳米粒可引起细胞内的活性氧增加,2、4、100μg·m L~(-1)PS纳米粒可引起活性氧降低;4、100μg·m L~(-1)PS-NH_2纳米粒可引起细胞内活性氧降低;0.1、1、2、4、100μg·m L~(-1)PS-COOH纳米粒可引起细胞内活性氧降低。结论低浓度PS纳米粒可增加HK-2细胞内的活性氧水平,高浓度则减少活性氧;PS-COOH纳米粒的活性氧降低效应比PS-NH_2强。
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