地榆皂苷Ⅱ抑制肿瘤细胞增殖和诱导其凋亡的作用
Proliferation inhibition and apoptosis induced by ziyuglycoside Ⅱ in cancer cells
作者：王振龙;何霞;成明;陈欣;姬建新;张强;陈爽;
Author：
收稿日期： 年卷（期）页码：2017,32(05):-485-488
期刊名称：华西药学杂志
Journal Name：WEST CHINA JOURNAL OF PHARMACEUTICAL SCIENCES
关键字：地榆皂苷Ⅱ;肿瘤;增殖抑制;细胞凋亡;Bcl-2蛋白家族;死亡受体;线粒体通路;内质网通路
Key words：
基金项目：国家自然科学基金资助项目(批准号:21172213;21302109;21402184;21572217)
中文摘要
目的检测地榆皂苷II对多种肿瘤细胞的体外增殖抑制作用,研究地榆皂苷II诱导肿瘤细胞凋亡的分子机制。方法采用SRB法检测地榆皂苷II对肿瘤细胞的体外增殖抑制,通过Western blot来研究地榆皂苷II对三大凋亡通路的影响。结果地榆皂苷Ⅱ可上调MDA-MB-231细胞中活性氧的水平,上调CHOP的表达,激活内质网应激途径;下调Bcl-2的表达,上调Bax的表达,激活线粒体凋亡途径;上调DR4、DR5的表达,激活TRAIL外源凋亡通路。结论地榆皂苷II通过激活三大细胞凋亡通路来抑制肿瘤细胞的增殖,促进肿瘤细胞的凋亡。
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