人肠道菌群对松果菊苷体外代谢转化的研究
Study on the metabolism of echinacoside by human intestinal bacteria in vitro
作者：钱浩;杨振亚;刘丹丹;张有龙;喻芳君;马志国;
Author：
收稿日期： 年卷（期）页码：2017,32(06):-641-643
期刊名称：华西药学杂志
Journal Name：WEST CHINA JOURNAL OF PHARMACEUTICAL SCIENCES
关键字：松果菊苷;肉苁蓉;人肠道菌群;体外;固相萃取;厌氧;代谢物;高效液相色谱-质谱联用法;电喷雾离子
Key words：
基金项目：国家自然科学基金资助项目(批准号:30902001)
中文摘要
目的研究人肠道菌群对松果菊苷代谢转化的情况。方法在体外厌氧条件下,将松果菊苷与人肠道菌群一起孵育不同时间,孵育液经固相萃取后,采用HPLC-ESI-MS法检测松果菊苷及其代谢物。结果孵育3 h后,孵育液中可检测到松果菊苷原型及毛蕊花糖苷、异毛蕊花糖苷、chamaedroside等主要代谢产物。但随着孵育时间的延长,松果菊苷及其代谢物均逐渐减少,9 h后几乎检测不到。结论在离体条件下,松果菊苷可被人肠道菌群代谢,主要代谢产物为毛蕊花糖苷、异毛蕊花糖苷、chamaedroside。
参考文献
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