大黄素促进结肠癌细胞自噬性凋亡的作用及其机制
Study on the promote effects and the mechanism of emodin on autophagy related-apoptosis in colon cancer cells
作者：李灵玲;蒲吉;
Author：
收稿日期： 年卷（期）页码：2017,32(06):-603-606
期刊名称：华西药学杂志
Journal Name：WEST CHINA JOURNAL OF PHARMACEUTICAL SCIENCES
关键字：结肠癌;人结肠癌细胞SW480;大黄素;自噬;凋亡;活性氧;细胞增殖;机制
Key words：
基金项目：
中文摘要
目的研究大黄素促进结肠癌细胞自噬性凋亡的作用机制。方法通过MTT法及Hochest染色法来检测大黄素对结肠癌细胞增殖及凋亡的影响;用免疫荧光检测自噬体的形成,用免疫印迹法检测自噬相关标志性蛋白的表达、细胞凋亡相关标志蛋白p53及caspase-3的活化及表达情况;用流式细胞仪检测胞内活性氧(ROS)的表达。结果大黄素对结肠癌细胞具明显的抑制作用且呈浓度依赖性,并可诱发肿瘤细胞凋亡;大黄素可促进结肠癌细胞自噬体形成、诱发细胞自噬;还可激活胞内ROS的表达。自噬抑制剂氯喹及ROS抑制剂NAC均能显著诱发大黄素诱导的促凋亡蛋白caspase-3和p53的活化表达。结论大黄素通过促进结肠癌细胞胞内的ROS表达来激活细胞自噬,进而抑制结肠癌细胞的增殖。
参考文献
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