丹参联合5-Fu对小鼠Lewis肺癌中HIF-1α、VEGF和MVD表达的影响
Effects of the expression of HIF- 1α,VEGF and MVD of Lewis lung carcinoma in mice of Danshen united 5- Fu
作者：冯俭;谢萍;秦银花;孙川;张煜华;张梦云;杨张婧;莫小宇;
Author：
收稿日期： 年卷（期）页码：2014,29(01):-42-44
期刊名称：华西药学杂志
Journal Name：WEST CHINA JOURNAL OF PHARMACEUTICAL SCIENCES
关键字：丹参;5-氟尿嘧啶;低氧诱导因子;血管内皮生长因子;微血管密度
Key words：
基金项目：2008年四川省科技厅应用基础研究项目(No.2008JY0008)
中文摘要
目的通过检测丹参联合5-Fu对小鼠Lewis肺癌低氧诱导因子-lα(HIF-lα)、血管内皮生长因子(VEGF)浓度和微血管密度(MVD),分析丹参对实体肿瘤局部血供和乏氧细胞氧压的影响,探讨丹参配合5-Fu提高疗效的机理。方法建立Lewis肺癌荷瘤鼠模型后,将60只小鼠随机均分为模型组、丹参低剂量组(丹低组)、丹参高剂量组(丹高组)、5-Fu组、丹参低剂量+5-Fu组(丹低+5-Fu组)、丹参高剂量+5-Fu组(丹高+5-Fu组)。于接种第2天起每天ip给药各组大鼠,末次给药24 h后检测肿瘤细胞中HIF-lαmRNA表达值、血清VEGF浓度和MVD。结果 5-Fu组、丹高+5-Fu组较模型组中HIF-lαmRNA的表达值降低(P
参考文献
[1]Weidner N.Review of current application in tumor prognostication[J].Semin Diagn Pathol,1993,10:302.
[2]王宜宗.肿瘤乏氧检测方法研究进展[J].肿瘤防治研究,2007,34(9):724-726.
[3]Semenza GL,Nejfelt MK,Chi SM,et al.Hypoxia-inducible nuclear factors bind to and enhancer element located to the human erythropoietin gene[J].Proc Natl Acad Sci USA,1991,88:5680-5684.
[4]Vaupel P,Thews O,Hoeckel M.Treatment resistance of solid tumors role of hypoxia and anemia[J].Med Oncol,2001,18(4):243-259.
[5]Shweiki D,Neeman M,Itrin A,et al.Induction of vascular endothelial growth factor expression by hypoxia and by glucose deficiency unmulticell spheorids Implications for tumor angiogenesis[J].Porc Natl Acad Sci USA,1995,92(3):768-772.
[6]Damert A,Machein M,Breier G,et al.Up-regulation of vascular endothelial growth factor expression in a rat glioma is conferred by two distinct hypoxia-driven mechanisms[J].Cancer Res,1997,57(17):3860-3864.
[7]Bremnes RM,Camps C,Sirem R.The prognostic impact of neoangiogenesis and the cytokines VEGF and bFGF in tumors and blood[J].Lung Cancer,2006,51(2):143-158.
[8]Cheng AS,Chan HL,To KF,et al.Cyclooxygenase-2 pathway correlates with vascular endothelial growth factor expression and tumor angiogenesis in hepatitis B virus associated hepatocellular carcinomal[J].Int J Oncol,2004,24(4):853-860.
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