雷帕霉素抑制mTOR活性诱导细胞的生长和下调EBV~+人鼻咽癌细胞HIF-1α介导的VEGF表达
mTOR inhibition impacts cell viability and downregulates expression of HIF-1α-mediated VEGF in EBV-positive human nasopharyngeal carcinoma cells
作者：任源;黄建鸣;查晓;邓碧芳;郎锦义;
Author：
收稿日期： 年卷（期）页码：2013,28(01):-50-52
期刊名称：华西药学杂志
Journal Name：WEST CHINA JOURNAL OF PHARMACEUTICAL SCIENCES
关键字：雷帕霉素;哺乳动物雷帕霉素靶蛋白;鼻咽癌;乏氧诱导因子1α;血管内皮生长因子;EB病毒
Key words：
基金项目：四川省科技厅科研基金项目(编号:2008JY0020)
中文摘要
目的观察雷帕霉素(Rap)体外对人鼻咽癌HNE-1EBV+和CNE-1EBV-细胞乏氧诱导因子1α(HIF-1α)和血管内皮生长因子(VEGF)表达的影响及生长抑制作用。方法采用MTT法检测Rap作用72 h IC50;用RT-PCR和ELISA分别测定Rap作用24 h后,细胞HIF-1αmRNA和培养上清液中VEGF的表达水平。结果 Rap呈剂量依赖性地抑制HNE-1EBV+和CNE-1EBV-细胞的生长,IC50分别为43.9、41.7μmol.L-1;Rap使HNE-1EBV+细胞HIF-1αmRNA和VEGF表达水平分别下调了24%、55%,但对CNE-1EBV-细胞无明显影响。结论 Rap对两株鼻咽癌细胞具有相当的生长抑制作用,可显著下调HNE-1EBV+细胞HIF-1α介导的VEGF表达,提示Rap对于改善EBV+鼻咽癌的治疗预后具有潜在的作用。
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