新型氮芥衍生物XHH诱导K562细胞凋亡的研究
Research on apoptosis of K562 cells induced by a novel nitrogen mustard derivative XHH
作者：苗久旺;张忠泉;张亚宏;李骞;赵瑾;谢松强;王超杰;
Author：
收稿日期： 年卷（期）页码：2012,27(01):-5-8
期刊名称：华西药学杂志
Journal Name：WEST CHINA JOURNAL OF PHARMACEUTICAL SCIENCES
关键字：氮芥衍生物;抗肿瘤;凋亡;Caspases
Key words：
基金项目：国家自然科学基金资助项目(批准号:20872027);;
国家重大研究计划培育项目(No.90913001);;
中国博士后科学基金(No.20090450092);;
河南省高等学校青年骨干教师资助计划(2009GGJS-022)
中文摘要
目的探讨新型氮芥衍生物N-[4-(二氯乙基)丁胺]-1,8-萘酰亚胺(XHH)对K562细胞的抗肿瘤作用及其机制。方法采用MTT法检测XHH对K562细胞增殖的影响;用Annexin V-FITC/Hoechst33342、PI/Hoechst33342和Rh123/Hoechst33342双染法高内涵观察细胞的形态学变化、凋亡率及线粒体膜电位;用分光光度法检测caspase-3、caspase-9的活性。结果在一定浓度范围内,XHH能抑制K562细胞的增殖且抗肿瘤活性优于美法仑,呈剂量和时间依赖性地诱导细胞凋亡、降低线粒体膜电位、活化caspase-3和caspase-9。结论 XHH具有较好的抗肿瘤活性,可通过线粒体/caspase-9/caspase-3途径诱导K562细胞凋亡。
参考文献
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