LC-MS/MS法测定大鼠血浆中的利福布丁
Determination of Rifabutin in rats plasma by LC-MS/MS
作者：刘晓茜;姚亚敏;马芳;张丽军;
Author：
收稿日期： 年卷（期）页码：2012,27(02):-184-186
期刊名称：华西药学杂志
Journal Name：WEST CHINA JOURNAL OF PHARMACEUTICAL SCIENCES
关键字：利福布丁;液质色谱联用技术;液液萃取;血药浓度
Key words：
基金项目：王宝恩肝纤维化基金(编号:201006);;
中央高校基本科研业务费专项资金(编号:10FX114)
中文摘要
目的用LC-MS/MS法测定大鼠血浆中利福布丁的浓度。方法以替米沙坦为内标,加入0.2 mL血浆,用1 mL乙酸乙酯萃取,高速离心后上清液氮气吹干并用流动相复溶,取20μL进样检测。结果利福布丁的最低检测限为0.5 ng.mL-1,在0.3～9.6μg.mL-1内线性良好,低、中、高浓度的提取回收率均大于85%,批内、批间RSD低于10%,成功检测了大鼠血浆中利福布丁的血药浓度。结论所用方法灵敏度好、准确度高、分析时间短、样品处理更简便,适用于测定利福布丁的血药浓度。
参考文献
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