大蒜素对大鼠心室肌间隙连接蛋白43表达水平的影响
Effects of allicin on the expression of connexin43 isoforms in hyperlipemic rat's cardiac muscle
作者：刘富;
Author：
收稿日期： 年卷（期）页码：2014,29(02):-146-148
期刊名称：华西药学杂志
Journal Name：WEST CHINA JOURNAL OF PHARMACEUTICAL SCIENCES
关键字：大蒜素;高脂血症;间隙连接蛋白43;免疫印迹;免疫组织化学;激光共聚焦
Key words：
基金项目：黑龙江省卫生厅科学技术研究项目(编号:2011-239)
中文摘要
目的通过检测大蒜素治疗前后高脂血症大鼠心肌间隙连接蛋白43的表达,阐述大蒜素降血脂作用的分子机制。方法将50只Wistar大鼠均分为正常组、模型组、大蒜素高、中、低(100、50、25 mg·kg-1·d-1)剂量组,建立大鼠高脂血症模型。从尾静脉取血,测血清中的总胆固醇(TC)、甘油三酯(TG)、低密度脂蛋白(LDL)、高密度脂蛋白(HDL)、游离脂肪酸(NEFA)等血脂指标;利用RT-PCR技术、免疫组化方法和免疫印迹技术测定间隙连接蛋白43在正常、模型、给药3种情况下的差异。结果高脂血症大鼠血清中的TC、TG、LDL、NEFA含量升高(P
参考文献
[1]Lawson LD,Wang ZJ.Allicin and allicin-derived garlic compounds increase breath acetone through allyl methyl sulfide:Use in measuring allicin bioavailability[J].J Agric Food Chem,2005,53(6):1974-1983.
[2]Osman M,Adnan A,Salmah BN,et al.Allicin has significant effect on autoimmune anti-islet cell antibodies in type 1 diabetic rats[J].Pol J Pathol,2012,63(4):248-254.
[3]Bagiu RV,Vlaicu B,Butnariu M.Chemical composition and in vitro antifungal activity screening of the Allium ursinum L.(Liliaceae)[J].Int J Mol Sci,2012,13(2):1426-1436.
[4]Abramovitz D,Gavri S,Harats D,et al.Allicin-induced decrease in formation of fatty streaks(atherosclerosis)in mice fed a cholesterol-rich diet.[J].Coron Artery Dis,1999,10(7):515-519.
[5]Ezhov MV,Il'ina LN,Safarova MS,et al.Cascade plasma filtration during the first year after CABG in patients with hyperlipidemia refractory to statins[J].Atheroscler Suppl,2013,14(1):101-105.
[6]Chan JY,Yuen AC,Chan RY,et al.A review of the cardiovascular benefits and antioxidant properties of allicin[J].Phytother Res,2012 Aug 8.
[7]Ruscic KJ,Miceli F,Villalba-Galea CA,et al.IKs channels open slowly because KCNE1 accessory subunits slow the movement of S4 voltage sensors in KCNQ1 pore-forming subunits[J].Proc Natl Acad Sci USA,2013 Jan 28.
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