通络醒脑泡腾片对Aβ海马注射痴呆大鼠tau蛋白磷酸化关键调控蛋白CDK5和GSK-3表达的影响
Effects of Tongluo Xingnao effervescent tablet on expression of CKD5 and GSK-3 in rat model of hippocampus with Aβ injection
作者：徐世军;代渊;张荫杰;熊敏;马云桐;钟振东;
Author：
收稿日期： 年卷（期）页码：2013,28(02):-140-143
期刊名称：华西药学杂志
Journal Name：WEST CHINA JOURNAL OF PHARMACEUTICAL SCIENCES
关键字：通络醒脑泡腾片;学习记忆;海马;CDK5;GSK-3;阿尔茨海默病
Key words：
基金项目：“重大新药创制”科技重大专项(2013ZX09103002-008);;
四川省杰出青年学术技术带头人资助计划(2011JQ0014);;
四川省学术和技术带头人培养资金资助项目(重点);;
四川省"十一五"支撑项目(2010SZ0195);;
四川省教育厅自然科学重点项目(12ZA041);;
成都市产业发展技术支撑计划—高校院所应用基础研究(12DXYB321JH-002)
中文摘要
目的探讨通络醒脑泡腾片对AD大鼠学习记忆和海马CKD5和GSK-3表达的影响,为其防治阿尔茨海默病提供依据。方法建立Aβ25-35海马注射痴呆大鼠模型,采用Morris水迷宫检测大鼠学习记忆能力,采用免疫组织化学和图像分析技术定量分析大鼠海马CKD5和GSK-3表达。结果通络醒脑泡腾片能显著缩短训练第3天和第5天大鼠定向航行测试的逃避潜伏期,显著延长空间探索测试第一象限时间和有效区停留时间,缩短第三象限时间;能够显著降低海马区CDK5和GSK-3的表达,平均光密度值显著降低。结论通络醒脑泡腾片具有提高AD大鼠学习记忆能力,抑制海马CDK5和GSK-3表达的作用,提示其益智作用与抑制海马CDK5和GSK-3有关。
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