LC-MS/MS法测定大鼠血浆中的吴茱萸碱、吴茱萸次碱和吴茱萸内酯及其生物利用度的研究
Simultaneous determination and absolute oral bioavailability of evodiamine,rutaecarpine and evodin concentration in the rat plasma by LC-MS /MS
作者：姚迪;孙晶晶;刘雷;顾桂英;马博;张琪;佘金雄;
Author：
收稿日期： 年卷（期）页码：2014,29(03):-298-302
期刊名称：华西药学杂志
Journal Name：WEST CHINA JOURNAL OF PHARMACEUTICAL SCIENCES
关键字：吴茱萸碱;吴茱萸次碱;吴茱萸内酯;液相色谱串联质谱法;药代动力学;绝对生物利用度
Key words：
基金项目：国家自然科学基金青年资助项目(批准号:81302835);;
江苏省自然科学基金资助项目(BK20130951);;
江苏省临床医学科技专项(BL2013028)
中文摘要
目的采用LC-ESI-MS/MS法同时测定大鼠血浆中的吴茱萸碱、吴茱萸次碱和吴茱萸内酯,并研究这3种活性物质在大鼠体内的药动学特征。方法取100μL血浆样品,用甲基叔丁基醚-二氯甲烷(3∶1)萃取,选择右啡烷为内标。采用BDS Hypersil C18色谱柱(100 mm×2.1 mm,2.4μm),乙腈-2 mmol·L-1乙酸铵(含0.05%甲酸)(60∶40)为流动相,采用AB Sciex 4000 Q TRAP型三重四级杆串联质谱,ESI源,多反应监测(MRM)正离子模式。经检测,吴茱萸碱、吴茱萸次碱、吴茱萸内酯和内标的检测离子对分别为:m/z 304.3→134.4、m/z 288.1→273.3、m/z 471.3→425.2、m/z 258.0→201.0。结果血浆中吴茱萸碱、吴茱萸次碱和吴茱萸内酯的线性范围均为0.5~1×103ng·mL-1(r>0.9960),提取回收率均>70%,日内、日间RSD均
参考文献
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