丹参联合放疗对小鼠Lewis肺癌肿瘤组织微血管的影响
Influence of Danshen combined with radiotherapy on micro-vascular of tumor tissue in mice with Lewis lung carcinoma
作者：冯俭;谢萍;孙川;秦银花;李煌;莫小宇;
Author：
收稿日期： 年卷（期）页码：2012,27(03):-276-278
期刊名称：华西药学杂志
Journal Name：WEST CHINA JOURNAL OF PHARMACEUTICAL SCIENCES
关键字：丹参;放疗;抑瘤率;微血管密度
Key words：
基金项目：四川省科技厅应用基础研究项目(No.2008JY0008)
中文摘要
目的探讨丹参联合放疗对小鼠Lewis肺癌肿瘤组织微血管密度(MVD)的影响。方法建立Lewis肺癌荷瘤鼠模型后,60只荷瘤鼠随机均分为模型组、丹参组(低、高剂量)、放疗组、丹参低剂量+放疗组(联合1组)、丹参高剂量+放疗组(联合2组)。各组于接种第二日起每天ip给药,连续12 d,放疗组及联合1、2组于接种第7日起给予钴-60照射肿瘤局部,剂量2 Gy·d-1,连续5 d。末次给药24 h后处死动物,剥离肿瘤组织称重,计算抑瘤率;免疫组化法检测肿瘤组织MVD。结果联合1组的MVD较放疗组和丹参低剂量组明显降低(P
参考文献
[1]Shweiki D,Itrin A,Soffer D,et al.Vascular endothelial growthfactor induced by hypoxia may mediate hypoxia-mediated angio-genesis[J].Nature,1992,359(6398):843-845.
[2]Shweiki D,Neeman M,Itrin A,et al.Induction of vascular endo-thelial growth factor expression by hypoxia and by glucose defi-ciency unmulticell spheorids:Implications for tumor angiogenesis[J].Porc Natl Acad Sci USA,1995,92(3):768-772.
[3]徐亚东,张薇,屠溪琳,等.丹参对Lewis肺癌小鼠组织形态学及血液系统影响的研究[J].中国中医药科技,2008,15(5):400.
[4]Weidner N.Tumor Angiogenesis:Review of current application intumor prognostication[J].Semin Diagn Pathol,1993,10:302.
[5]Bremnes RM,Camps C,Sirem R.Angiogenesis in non-small celllung cancer:The prognostic impact of neoangiogenesis and thecytokines VEGF and bFGF in tumors and blood[J].Lung Canc-er,2006,51(2):143-158.
[6]陈洪雷,张苗,王雅杰,等.放疗对荷瘤鼠肿瘤血管生成的抑制作用[J].现代肿瘤医学,2006,14(12):1494.
[7]Jain RK.Normalization of tumor vasculature:An emerging conceptin antiangiogenic therapy[J].Sci,2005,307(5706):58-62.
[8]Ou GF,Hasaka S,Zeng LH,et al.U sefulness of H IF-1 ima-ging for determining optimal timing of combining bevacizumab andradiotherapy[J].Int J Radiation Oncology Physics,2009,75(2):463-467.
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