反式白藜芦醇对Aβ_(25-35)诱导的阿尔茨海默病大鼠的海马神经元的保护作用
Protective effects of trans-resveratrol on hippocampal neurons in the rats with Aβ_(25-35) induced Alzheimer's disease
作者：程青格;徐平;龚其海;陈群;史艳红;
Author：
收稿日期： 年卷（期）页码：2014,29(03):-278-281
期刊名称：华西药学杂志
Journal Name：WEST CHINA JOURNAL OF PHARMACEUTICAL SCIENCES
关键字：反式白藜芦醇;海马;学习;记忆;APP;Caspase 8;β淀粉样蛋白前体蛋白水解产物
Key words：
基金项目：贵州省科技厅社发攻关项目(黔科合SY字[2010]3074)
中文摘要
目的观察反式白藜芦醇(TR)对Aβ25-35诱导AD大鼠海马神经元的保护作用。方法将45只大鼠随机均分为假手术组、阳性对照组(多奈哌齐1 mg·kg-1)、模型组(Aβ25-35)及TR低、高剂量组(TR 10、40 mg·kg-1);Aβ25-35注射海马内后,各组ig给药,qd,连续15 d,假手术组和模型组给予同体积的生理盐水。Morris水迷宫检测大鼠学习和记忆能力;HE染色观察海马神经元损伤情况;实时PCR法定量检测海马中APP mRNA的表达;用免疫组化及Western Blot检测caspase8蛋白表达。结果与模型组比较,TR低、高剂量组在定向航行实验中,大鼠逃避潜伏期明显缩短(P
参考文献
[1]Xu F,Crande AM,Robinson JK,et al.Early onset subicular microvascular amyloid and neuroinflammation correlate with behavioral deficits in vasculotropic mutant amyloid beta-protein precursor transgenic mice[J].Neuros,2007,146(1):98-107.
[2]Pompl PN,Yemul S,Xiang Z,et al.Caspase gene expression in the brain as a function of clinical progression of Alzheimer’s disease[J].Arch Neurol,2003,60(3):369-376.
[3]Chen WP,Su MJ,Huang LM.In vitro electrophysiological mechanisms for antiarrhythmic efficacy of resveratrol,a red wine antioxidant[J].Eur J Pharmacol,2007,554(2):196-204.
[4]王顺旺,徐平,刘海军,等.反式白藜芦醇对Aβ25-35致痴呆大鼠海马诱导型一氧化氮表达的影响[J].重庆医学,2010,39(18):2400-2405.
[5]龚其海,丁利静,王丽娜,等.蛇麻子素减轻脂多糖诱导的大鼠学习记忆减退[J].中国新药与临床杂志,2011,30(8):609-614.
[6]李静,周华东,王延江,等.海马注射β淀粉样蛋白建立阿尔茨海默病大鼠模型的实验研究[J].中国临床神经学,2008,16(5):467-471.
[7]Diaz A,Mendieta L,Zenteno E,et al.The role of NOS in the impairment of spatial memory and damaged neurons in rats injected with Aβ25-35 into the temporal cortex[J].Pharmacol Biochem Behav,2011,98(1):67-75.
[8]Tomiyama T.Involvement of beta-amyloid in the etiology of Alzheimer's disease[J].Brain Nerve,2010,62(7):691-699.
[9]袁海峰,李玺,张智燕.脑尔康对AD模型小鼠脑内APP、Aβ表达的影响[J].中国实验方剂学杂志,2011,17(24):140-143.
[10]Pepeu G,Giovannini MG.Cholinesterase inhibitors and beyond[J].Curr Alzheimer Res,2009,6(2):86-96.
[11]Kamat PK,Tota S,Shukla R,et al.Mitochondrial dysfunction:A crucial event in okadaic acid(ICV)induced memory impairment and apoptotic cell death in rat brain[J].Pharmacol Biochem Behav,2011,100(2):311-319.
【关闭】
