莴苣苷B对缺血性脑卒中大鼠海马VEGF mRNA及蛋白表达的影响
Effects of lactuside B on the expression of VEGF mRNA and protein in the rats' hippocampus after ischemic stroke
作者：李生莹;张海芳;詹合琴;闫福林;杨宇平;崔泰震;
Author：
收稿日期： 年卷（期）页码：2014,29(03):-266-269
期刊名称：华西药学杂志
Journal Name：WEST CHINA JOURNAL OF PHARMACEUTICAL SCIENCES
关键字：莴苣苷B;缺血性脑卒中;海马;VEGF;基因;蛋白
Key words：
基金项目：国家自然科学基金资助项目(批准号:81172953);;
河南省教育厅自然科学研究项目(2009A310009)
中文摘要
目的探讨高翅果菊提取物莴苣苷B对缺血性脑卒中大鼠海马中VEGF mRNA及其蛋白表达的影响。方法将SD大鼠随机分为6组,即假手术组、模型组、阳性对照组、莴苣苷B低、中、高(12.5、25、50 mg·kg-1)剂量组,大鼠均按5 mL·kg-1ip给药,bid。各组于给药24 h后处死7只小鼠,其中4只用于免疫组化研究,3只用于RT-PCR研究,剩余小鼠于给药72 h后处死,每组的4只用于免疫组化研究,其他用于RT-PCR研究。结果再灌注24 h时模型组海马中VEGF的基因和蛋白表达增多,与假手术组比较有显著性差异(P
参考文献
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