脂质体-鱼精蛋白-DNA复合物的制备及对树突状细胞的成熟诱导
Preparation of liposomes-protamine-DNA complexes for maturation induction on dendritic cells
作者：李攀;陈思穆;龚涛;张志荣;孙逊;
Author：
收稿日期： 年卷（期）页码：2013,28(03):-246-248
期刊名称：华西药学杂志
Journal Name：WEST CHINA JOURNAL OF PHARMACEUTICAL SCIENCES
关键字：脂质体-鱼精蛋白-DNA复合物;树突状细胞;成熟诱导
Key words：
基金项目：
中文摘要
目的研究脂质体-鱼精蛋白-DNA复合物(LPD)的制备方法及对树突状细胞(DC2.4)的成熟诱导作用。方法薄膜-超声分散法制备空白的阳离子脂质体,再与鱼精蛋白-DNA复合物室温孵育形成LPD;测定其粒径和电位。流式细胞仪检测鼠源树突状细胞系DC2.4的甘露糖受体表达水平;用DC2.4表面标记分子CD80、CD40、CD86和MHC-2的表达水平,考查LPD对树突状细胞的成熟诱导作用。结果 LPD的最佳比例为DDAB-鱼精蛋白-DNA(2∶1.5∶1);LPD粒径为84.28±0.56 nm,Zeta电位为27.33±1.23 mV。通过FITC-ManBSA的结合作用检测到DC2.4表面有83%的甘露糖受体表达。LPD明显上调DC2.4表面标记分子的表达水平(P
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