PEG修饰的紫杉醇固体脂质纳米粒的制备及其性质研究
Preparation and characters investigation of PEG modified paclitaxel solid lipid nanoparticles
作者：郑洁;万瑜;樊戈睿;孙逊;
Author：
收稿日期： 年卷（期）页码：2014,29(03):-234-236
期刊名称：华西药学杂志
Journal Name：WEST CHINA JOURNAL OF PHARMACEUTICAL SCIENCES
关键字：PEG修饰;紫杉醇;固体脂质纳米粒;苦味酸-分光光度法
Key words：
基金项目：
中文摘要
目的制备PEG修饰的固体脂质纳米粒,并研究其性质。方法采用薄膜超声法制备包载紫杉醇的固体脂质纳米粒,再利用EDC/NHS脱水缩合法在纳米粒表面进行PEG修饰,得到PEG修饰的固体脂质纳米粒。用苦味酸-分光光度法测定PEG的含量;用透射电镜考察纳米粒的形态特点;采用凝胶柱洗脱联合HPLC测定包封率;筛选纳米粒的最佳冻干条件。结果成功制备了PEG修饰的紫杉醇固体脂质纳米粒,通过脱水缩合法连接到纳米粒上的PEG化多肽占加入总量的26.5%;紫杉醇固体脂质纳米包封率为75%,载药量为7%。结论制备的PEG修饰的紫杉醇固体脂质纳米粒具有较整齐的外观形态、较高载药量和较好的稳定性。
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