HPLC测定缩宫素原料药的含量
Determination of oxytocin by HPLC
作者：曹雨;孔毅;张聪颖;邵妤;康国伟;杨毅跃;杜迎翔;
Author：
收稿日期： 年卷（期）页码：2013,28(04):-412-414
期刊名称：华西药学杂志
Journal Name：WEST CHINA JOURNAL OF PHARMACEUTICAL SCIENCES
关键字：缩宫素;含量测定;高效液相色谱法
Key words：
基金项目：
中文摘要
目的采用HPLC测定缩宫素原料药的含量。方法色谱柱为Agilent Zorbax SB-C18(250 mm×4.6 mm,5μm),流动相A为15.6 g.L-1磷酸二氢钠,流动相B为50%乙腈,流速1.0 mL.min-1,检测波长220 nm,进样量25μL,外标法定量。结果缩宫素的线性范围为150～350μg.mL-1(r=0.9992);定量限为0.025μg.mL-1。结论所用方法简便、快速、准确,专属性好,可用于缩宫素原料药的含量测定。
参考文献
[1]刘倩,钱德明,刘群丽,等.缩宫素及其类似物的研究进展[J].药物分析杂志,2011,31(3):609-613.
[2]Hawe A,Poole R,Romeijn S,et al.Towards heat-stable oxytocinformulations:Analysis of degradation kinetics and identification ofdegradation products[J].Pharm Res,2009,26(7):1679-1688.
[3]Kosfeld M,Heinrichs M,Zak PJ,et al.Oxytocin increases trust inhumans[J].Nature,2005,435(2):673-676.
[4]European Directorate for the Quality of Medicines.EuropeanPharmacopoeia,7th ed[S].Strasbourg,France:European Directo-rate for the Quality of Medicines,2011:2653.
[5]United States Pharmacopeial Convention.United States Pharmaco-peia,34st ed[S].Rockville,MD,USA:United States Pharmaco-peial Convention,2011:647.
[6]中华人民共和国国家药典委员会.中国药典[S].北京:中国医药科技出版社,2010:1106-1107.
[7]Ashenafi D,Hemelrijck EV,Chopra S,et al.Liquid chromato-graphic analysis of oxytocin and its related substances[J].JPharm Biomed Analy,2010,51:24-29.
【关闭】
