甘草次酸对谷氨酸所致PC12细胞损伤的保护作用及其机理
The involvement of mitochondria-mediated pathway in protective effect of 18β-glycyrrhe-tinic acid against glutamate-induced PC12 cells apoptosis
作者：王立英;刘雨萌;金元宝;赵明智;夏婷;孟凡欣;
Author：
收稿日期： 年卷（期）页码：2013,28(04):-353-356
期刊名称：华西药学杂志
Journal Name：WEST CHINA JOURNAL OF PHARMACEUTICAL SCIENCES
关键字：甘草次酸;谷氨酸;PC12细胞;凋亡;线粒体功能
Key words：
基金项目：
中文摘要
目的考察甘草次酸对谷氨酸诱导的PC12细胞损伤的保护作用及作用机制。方法使用20 mmol.L-1谷氨酸处理大鼠嗜铬细胞瘤细胞株(PC12),构建细胞凋亡模型。采用药物预处理给药方法,MTT法测定细胞存活率,Fluo-4 AM染色测定胞内钙离子浓度,JC-1染色测定线粒体跨膜电位变化,western blot测定Bcl-2和Bcl-Xl蛋白表达。结果甘草次酸可明显提高谷氨酸诱导的PC12细胞的还原能力,抑制细胞LDH的释放,提高细胞存活率;抑制兴奋性谷氨酸所引起的细胞内钙离子内流;还原谷氨酸引起的线粒体跨膜电位的减弱,增加Bcl-2和Bcl-Xl蛋白的表达量。结论甘草次酸可通过线粒体依赖途径有效抑制谷氨酸诱导的PC12细胞凋亡。
参考文献
[1]Traynelis SF,Wollmuth LP,McBain CJ,et al.Glutamate recep-tor ion channels:Structure,regulation,and function[J].Pharma-col Rev,2010,62(3):405-496.
[2]Reynolds IJ,Hastings TG.Glutamate induces the production of re-active oxygen species in cultured forebrain neurons following NM-DA receptor activation[J].J Neurosci,1995,15(5):3318-3327.
[3]Agarwal MK,Iqbal M,Athar M.Inhibitory effect of 18beta-gly-cyrrhetinic acid on 12-O-tetradecanoyl phorbol-13-acetate-induced cutaneous oxidative stress and tumor promotionin mice[J].Redox Rep,2005,10(3):151-157.
[4]Kao TC,Shyu MH,Yen GC.Neuroprotective effects of glycyrrhi-zic acid and 18beta-glycyrrhetinic acid in PC12 cells via modu-lation of the PI3K/Akt pathway[J].J Agric Food Chem,2009,57(2):754-761.
[5]吴南,冯华,朱刚,等.谷氨酸毒性损伤致PC12细胞NMDAR与CB表达的变化[J].重庆医学,2003,32(2):137-139.
[6]Choi DW.Calcium and excitotoxic neuronal injury[J].Ann N YAcad Sci,1994,747:162-171.
[7]Chao DT,Korsmeyer SJ.BCL-2 family:Regulators of cell death[J].Annu Rev Immuno,l998,16:395-419.
[8]Raisova M,Hossini AM,Eberle J,et al.The Bax/Bcl-2 ratiodetermines the susceptibility of human melanoma cells to CD95/Fas-mediated apoptosis[J].J Invest Dermatol,2001,117(2):333-340.
【关闭】
