HPLC测定复方盐酸二甲双胍格列美脲胶囊中的格列美脲
Determination of glimepiride in Complex metformin hydrochloride-glimepiride capsules
作者：白靖;曹亮;张倩;方瑜;曹德英;
Author：
收稿日期： 年卷（期）页码：2012,27(04):-438-440
期刊名称：华西药学杂志
Journal Name：WEST CHINA JOURNAL OF PHARMACEUTICAL SCIENCES
关键字：盐酸二甲双胍;格列美脲;高效液相色谱法;含量
Key words：
基金项目：
中文摘要
目的用HPLC法测定复方盐酸二甲双胍格列美脲胶囊中格列美脲的含量。方法采用Diamonsil C18色谱柱(250mm×4.6 mm,5μm),流动相为0.05 mol·L-1甲酸铵缓冲液(pH4)-乙腈(40∶60),检测波长228 nm,流速1.0 mL·min-1。结果 1.014～20.280μg·mL-1格列美脲与峰面积呈良好的线性关系(r=0.9999),平均回收率为99.5%,RSD=1.0%。结论所用方法简便、准确性高、专属性强,且盐酸二甲双胍对格列美脲的测定无干扰,可用于该类复方制剂的质量控制。
参考文献
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