小分子干扰RNA-紫杉醇固体脂质纳米粒的制备
Preparation of the solid lipid nanoparticles of siRNA and paclitaxel
作者:姚欣宇;石三军;孙逊;
Author:
收稿日期: 年卷(期)页码:2014,29(04):-368-370
期刊名称:华西药学杂志
Journal Name:WEST CHINA JOURNAL OF PHARMACEUTICAL SCIENCES
关键字:固体脂质纳米粒;凝胶柱层析法;紫杉醇;小分子干扰RNA;高效液相色谱法
Key words:
基金项目:
中文摘要
目的制备包裹小分子干扰RNA(siRNA)-紫杉醇(PTX)的固体脂质纳米粒(SLNs)(siRNA-PTX-SLNs),考察其理化性质和包封率。方法采用薄膜超声法制备siRNA-PTX-SLNs;凝胶柱层析法分离SLNs、游离的PTX及游离的siRNA;HPLC法检测PTX的含量;荧光标记法检测siRNA的含量;测定粒径、电位,并在电镜下观察形状。结果 siRNA-PTX-SLNs中紫杉醇的包封率>96%;siRNA的包封率为56.65%±0.5%;SLNs的粒径、电位分别为155±0.23 nm、40±2.34 mV。结论成功制备了能同时有效包裹siRNA和PTX的siRNA-PTX-SLNs。
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