17β-雌二醇抗心肌细胞氧化应激损伤的Nrf2/HO-1信号通路的分子机制
17β-estradiol protects H_9C_2 cardiomyocytes from H_2O_2-induced injury via Nrf2/HO-1 signaling pathway
作者：杨静;黎洁;卢长喜;郭爱华;刘长青;杨华;石慧武;牛若颖;
Author：
收稿日期： 年卷（期）页码：2013,28(05):-467-470
期刊名称：华西药学杂志
Journal Name：WEST CHINA JOURNAL OF PHARMACEUTICAL SCIENCES
关键字：H2O2;H9C2细胞;17β-雌二醇;Nrf 2;HO-1;氧化应激
Key words：
基金项目：
中文摘要
目的观察17β-雌二醇(E2)对H2O2诱导的心肌细胞氧化应激损伤的保护作用,探讨其可能的分子机制。方法将细胞分为对照组、H2O2组、E2组、抑制剂组。用MTT法观察心肌细胞的活性;用Western Blot技术检测Nrf 2的蛋白表达;用RT-PCR技术观察Nrf 2下游靶基因HO-1的mRNA水平。结果 100μmol·L-1H2O2可使H9C2细胞活性显著下降,E2预处理可有效阻止H2O2诱导的心肌细胞损伤;Nrf 2的蛋白表达自E2和H2O2共同培育2 h时即显著高于对照组,6 h达到最高峰;E2也诱导了H2O2处理后H9C2细胞HO-1 mRNA水平及其蛋白表达的升高;ICI 182,780明显逆转了E2的心肌保护作用。结论 E2可有效降低H2O2诱导的心肌细胞损伤,Nrf 2/HO-1信号通路的上调可能是其重要的分子机制。
参考文献
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