人参皂苷Rb1抗血管平滑肌细胞增殖与calcineurin通路及抗氧化作用的关系
The anti-proliferative effect of ginsenoside Rb1 on vascular smooth muscle cells and its relationship with calcineurin signaling and anti-oxidant effect
作者：张纾;高杨;邓江;黄燮南;
Author：
收稿日期： 年卷（期）页码：2013,28(05):-461-463
期刊名称：华西药学杂志
Journal Name：WEST CHINA JOURNAL OF PHARMACEUTICAL SCIENCES
关键字：人参皂苷Rb1;血管平滑肌细胞增殖;钙调神经磷酸酶信号通路;抗氧化作用
Key words：
基金项目：贵州省优秀科技教育人才省长专项基金项目(编号:2007-53)
中文摘要
目的观察人参皂苷Rb1(Rb1)抗血管平滑肌细胞增殖效应与钙调神经磷酸酶(CaN)信号通路的关系,并探讨该药抗增殖效应是否与其抗氧化作用有关。方法采用球囊导管损伤血管内皮造成大鼠颈动脉内膜增生,ip Rb1两周后,取血样测定过氧化物歧化酶(SOD)活性及丙二醛(MDA)含量变化;在培养的血管平滑肌细胞(VSMC)中加入血小板衍化生长因子-BB(PDGF-BB)诱导其增殖,给予Rb1 24 h后,观察VSMCs增殖和CaN mRNA表达的改变。结果 10、30 mg·kg-1·d-1Rb1给药两周使球囊损伤而变窄的大鼠颈动脉管腔面积明显扩大,伴有SOD活性升高和血中MDA水平的降低;在培养VSMCs中,加入抗增殖浓度Rb1(25、50、100μg·mL-1)后,显著下调PDGF-BB所致CaN mRNA的过度表达。结论 Rb1抗VSMC增殖效应至少部分与其抗氧化作用有关,并可能涉及其对CaN通路的抑制。
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