PEG修饰的腺病毒-阴离子脂质体复合物的制备及分离纯化
Preparation and purification of PEG modified adenovirus-anionic liposomes complexes
作者：万瑜;樊戈睿;郑洁;孙逊;
Author：
收稿日期： 年卷（期）页码：2013,28(05):-444-446
期刊名称：华西药学杂志
Journal Name：WEST CHINA JOURNAL OF PHARMACEUTICAL SCIENCES
关键字：PEG;腺病毒;阴离子脂质体;蔗糖密度梯度离心
Key words：
基金项目：国家科技重大专项[编号:2011ZX09401-304(4-3)]
中文摘要
目的制备并分离纯化PEG修饰的腺病毒-阴离子脂质体复合物。方法采用薄膜分散法制备空白阴离子脂质体,利用钙离子融合法制备阴离子脂质体与腺病毒的复合物,最后用后插入法制备PEG修饰的腺病毒-阴离子脂质体复合物。通过蔗糖密度梯度离心法分离制备的复合物,分别测定分离得到的各组分中脂质体、PEG-脂质材料及腺病毒的含量。结果成功制备了PEG修饰的腺病毒-阴离子脂质体复合物,连接到脂质体上的PEG-脂质占加入总量的28.9%,被脂质体包裹的腺病毒占加入量的35.9%。结论通过蔗糖密度梯度离心可得到纯化的PEG修饰的腺病毒-阴离子脂质体复合物。
参考文献
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