RP-HPLC测定人血液和尿液中的倍他米松
Determination of Betamethasone in the blood and urine by RP-HPLC
作者：盛欣;廖林川;颜有仪;杨林;刘晓敏;詹兰芬;王智慧;
Author：
收稿日期： 年卷（期）页码：2011,26(02):-179-181
期刊名称：华西药学杂志
Journal Name：WEST CHINA JOURNAL OF PHARMACEUTICAL SCIENCES
关键字：倍他米松;高效液相色谱法;血液;尿液
Key words：
基金项目：
中文摘要
目的采用HPLC法测定人血液和尿液中的倍他米松。方法以乙酸乙酯为萃取剂、泼尼松为内标。用Welchrom C18柱,甲醇-水(60∶40)为流动相,检测波长240 nm。结果倍他米松在血液和尿液中的线性范围为0.1～20μg.mL-1(r2>0.999);检测限均为0.02μg.mL-1;平均方法回收率为91%～104%;日内、日间RSD≤4.6%;萃取回收率≥86.3%。结论所建方法准确、灵敏、简便,适用于血液、尿液中倍他米松的分析。
参考文献
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